Molecular Cancer Gene Expression Profiles in Primary Pancreatic Tumors and Metastatic Lesions of Ela-c-myc Transgenic Mice
Molecular Cancer Gene Expression Profiles in Primary Pancreatic Tumors and Metastatic Lesions of Ela-c-myc Transgenic Mice
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发表时间：
2008
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通讯作者：
A. Thakur;Aliccia B. Bollig;Jiusheng Wu;D. Liao;Jiusheng;J. Liao
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作者：
A. Thakur;Aliccia B. Bollig;Jiusheng Wu;D. Liao;Jiusheng;J. Liao
背景：胰腺癌通常是一种无法治愈的致命疾病，主要是由于其在诊断前的侵袭和转移所致。我们分析了来自Ela-c-myc转基因小鼠的成对的胰腺原发肿瘤和转移灶的基因表达谱，以确定可能与胰腺癌进展有关的基因。对筛选出的差异表达基因进行半定量和定量RT-PCR验证。为了进一步评估一些差异表达基因的相关性，我们研究了它们在高转移潜能和低转移潜能的人胰腺癌细胞系中的表达模式。
Background: Pancreatic carcinoma usually is a fatal disease with no cure, mainly due to its invasion and metastasis prior to diagnosis. We analyzed the gene expression profiles of paired primary pancreatic tumors and metastatic lesions from Ela-c-myc transgenic mice in order to identify genes that may be involved in the pancreatic cancer progression. Differentially expressed selected genes were verified by semi-quantitative and quantitative RT-PCR. To further evaluate the relevance of some of the selected differentially expressed genes, we investigated their expression pattern in human pancreatic cancer cell lines with high and low metastatic potentials.