The Influence of Age and Sex on Ocular Surface Microbiota in Healthy Adults
The Influence of Age and Sex on Ocular Surface Microbiota in Healthy Adults
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年龄和性别对健康成年人眼表微生物群的影响
DOI：
10.1167/iovs.17-22957
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发表时间：
2017-12-01
影响因子：
4.4
通讯作者：
Wei, Lai
中科院分区：
文献类型：
--
作者：
Wen, Xiaofeng;Miao, Li;Wei, Lai
目的。越来越多的证据表明，眼表微生物群具有强大的免疫调节功能，在健康眼睛的生理维持和眼部疾病的发病机制中发挥着关键作用。尽管已知微生物群受到年龄和性别的影响，但这些因素对健康成年人眼表微生物群的影响仍然很大程度上未知。眼表微生物组样本取自中山眼科中心 48 名年轻人和 42 名老年人的下球结膜。使用宏基因组鸟枪法测序，我们描述了健康成年人结膜微生物组特征的性别和年龄差异。结果。男性和女性群体仅在细菌群落的3种多样性上存在差异，而年轻群体和老年群体在细菌组成、代谢功能和抗生素耐药基因丰度方面存在显着差异。结论。我们的研究结果表明，年龄和性别共同塑造了结膜微生物组，并可能通过改变共生微生物组来改变眼表的免疫稳态。
PURPOSE. A growing body of evidence suggests that the microbiome of the ocular surface confers potent immunoregulatory functions and has a key role in the physiologic maintenance of healthy eyes and in the pathogenesis of ocular diseases. Although the microbiome is known to be affected by age and sex, the influence of these factors on ocular surface microbiota in healthy adults remains largely unknown.METHODS. Ocular surface microbiome samples were obtained from the inferior bulbar conjunctiva of 48 young and 42 old adults at Zhongshan Ophthalmic Center. Using metagenomic shotgun sequencing, we characterized the sex-and age-differences in conjunctival microbiome profiles of healthy adults.RESULTS. Male and female groups differed only in the 3 diversity of bacterial communities, while there were significant differences in bacterial composition, metabolic functions, and the abundance of antibiotic resistance genes between young and old adult groups.CONCLUSIONS. Our findings suggest that age and sex collectively shape the conjunctival microbiome, and may change the immune homeostasis of the ocular surface through alterations of its commensal microbiome.