ACTIVATION OF HUMAN-BLOOD MONOCYTES BY ADHERENCE TO TISSUE-CULTURE PLASTIC SURFACES
ACTIVATION OF HUMAN-BLOOD MONOCYTES BY ADHERENCE TO TISSUE-CULTURE PLASTIC SURFACES
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DOI：
10.1016/0014-4800(87)90049-9
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发表时间：
1987-06-01
影响因子：
3.6
通讯作者：
SCHWARTZ, CJ
中科院分区：
文献类型：
--
作者：
KELLEY, JL;ROZEK, MM;SCHWARTZ, CJ
将通过逆流离心淘洗纯化的人单核细胞在塑料皿或Teflon小瓶中培养，以确定附着是否会导致活化。β-测定葡萄糖醛酸酶活性、5“-核苷酸酶活性、纤溶酶原激活物和超氧阴离子生成作为单核细胞活化的标志物。在2、4、8和10小时测定条件培养基和细胞裂解物，然后每天测定，持续6天。在塑料培养皿中培养的单核细胞分泌显著更大比例的它们的β-葡萄糖醛酸苷酶进入培养基中的量比在特氟隆小瓶中培养的量多。在塑料培养皿中培养的单核细胞中，5“-核苷酸酶的活性较低，与较高的活化一致。细胞纤溶酶原激活剂水平和超氧阴离子产生的能力，提高在塑料培养皿中培养的细胞，相对于在聚四氟乙烯小瓶中培养的单核细胞。这些观察结果表明，单核细胞附着在塑料表面的结果，在他们的激活，一种现象，可能会影响的性质和解释的实验数据来自培养的粘附单核细胞或巨噬细胞。
Human monocytes, purified by countercurrent centrifugal elutriation, were cultured either in plastic dishes or in Teflon vials to determine if attachment would result in activation. .beta.-Glucuronidase activity, 5''-nucleotidase activity, plasminogen activator, and superoxide anion generation were measured as markers of monocyte activation. Conditioned media and cell lysates were assayed at 2, 4, 8, and 10 hr and then daily for 6 days. Monocytes cultured in plastic dishes secreted a significantly greater proportion of their .beta.-glucuronidase into the medium than those cultured in Teflon vials. The activity of 5''-nucleotidase was lower in monocytes cultured in plastic dishes, consistent with greater activation. Cellular plasminogen activator levels and the capacity for superoxide anion generation were enhanced in cells cultured in plastic dishes, relative to monocytes cultured in Teflon vials. These observations indicate that monocyte attachment in plastic surfaces results in their activation, a phenomenon that may influence the nature and interpretation of experimental data derived from cultured adherent monocytes or macrophages.