Comparative analyses of hypothalamus transcriptomes reveal fertility-, growth-, and immune-related genes and signal pathways in different ploidy cyprinid fish
Comparative analyses of hypothalamus transcriptomes reveal fertility-, growth-, and immune-related genes and signal pathways in different ploidy cyprinid fish
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DOI：
10.1016/j.ygeno.2021.01.004
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发表时间：
2021-01-27
期刊：
影响因子：
4.4
通讯作者：
Liu, Shaojun
中科院分区：
文献类型：
--
作者：
Li, Shengnan;Zhou, Yi;Liu, Shaojun
三倍体鲫鱼（Triploid crucian carp， TCC）是由雌性二倍体红鲫（Carassius auratus red var.， RCC）与雄性异源四倍体杂交种杂交而成。本研究采用高通量测序技术对二倍体RCC、二倍体鲤鱼（Cyprinus carpio L.， CC）和TCC的雌性下丘脑进行转录组分析。通过功能基因注释和差异基因表达筛选，获得下丘脑的关键功能表达基因。通过测序和基因组作图，分别获得71.56 G数据和47,572个基因。通过模糊聚类分析将差异表达基因（deg）划分为8组，对TCC中差异表达基因（6005,12.62%）和差异表达基因（3849,8.09%）进行进一步研究。KEGG富集分析表明，显性基因主要富集于4条途径。几个生育相关基因在TCC中表达水平较低，而几个生长相关基因和免疫相关基因在TCC中表达水平较高。并对15个deg进行了实时荧光定量PCR （qPCR）验证。本研究可为利用远缘杂交选育不育、速生、抗病品种提供参考。
Triploid crucian carp (TCC) is obtained by hybridization of female diploid red crucian carp (Carassius auratus red var., RCC) and male allotetraploid hybrids. In this study, high-throughput sequencing was used to conduct the transcriptome analysis of the female hypothalamus of diploid RCC, diploid common carp (Cyprinus carpio L., CC) and TCC. The key functional expression genes of the hypothalamus were obtained through functional gene annotation and differential gene expression screening. A total of 71.56 G data and 47,572 genes were obtained through sequencing and genome mapping, respectively. The Fuzzy Analysis Clustering assigned the differentially expressed genes (DEGs) into eight groups, two of which, overdominance expression (6005, 12.62%) and underdominance expression (3849, 8.09%) in TCC were further studied. KEGG enrichment analysis showed that the DEGs in overdominance were mainly enriched in four pathways. The expression of several fertility-related genes was lower levels in TCC, whereas the expression of several growth-related genes and immune-related genes was higher levels in TCC. Besides, 15 DEGs were verified by quantitative real-time PCR (qPCR). The present study can provide a reference for breeding sterility, fast-growth, and disease-resistant varieties by distant hybridization.