Maternal obesity alters C19MC microRNAs expression profile in fetal umbilical cord blood
Maternal obesity alters C19MC microRNAs expression profile in fetal umbilical cord blood
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DOI：
10.1186/s12986-020-00475-7
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发表时间：
2020-07-06
影响因子：
4.5
通讯作者：
Ding, Zhide
中科院分区：
文献类型：
--
作者：
Jing, Jia;Wang, Yingjin;Ding, Zhide
背景：确定超重/肥胖孕妇是否改变了胎儿脐带血中的microRNA表达模式，这可能会影响后代的发育。方法采集34例超重/肥胖儿和32例正常体重儿产后脐血标本。下一代测序(NGS)分析了它们的miRNA表达模式。使用Miranda和TargetScan数据库预测miRNAs的目标基因，然后进行基因本体论(GO)和京都基因和基因组百科全书(KEGG)的分析以进行功能分组和通路分析。QRT-PCR验证了在NGS结果中发现的差异表达的miRNAs的一致性。结果新生儿体重与孕前体重指数呈正相关(r=0.48，P<；0.001)。在正常组和超重/肥胖组之间有108个miRNA的差异表达，这些靶基因在代谢途径中得到了丰富。超重/肥胖组有5个C19MC miRNAs(miR-516a-5p、miR-516b-5p、miR-520a-3p、miR-1323、miR-523-5p)表达上调。靶向浓缩分析表明，它们参与了胚胎后发育、脂肪和葡萄糖的动态平衡、T细胞分化和神经系统发育。结论超重/肥胖孕妇孕期胎儿循环中C19MC miRNA表达上调可能参与其子代多系统代谢途径的改变。
Background To determine if overweight/obese pregnant women have altered microRNA expression patterns in fetal umbilical cord blood that may affect the development of offspring. Methods Umbilical cord blood samples were obtained from the fetuses of 34 overweight/obese and 32 normal-weight women after delivery. Next generation sequencing (NGS) analyzed their miRNA expression patterns. miRanda and TargetScan databases were used to predict the miRNAs' target genes followed by analyses of Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) to perform function grouping and pathway analyses. qRT-PCR verified the identity of differentially expressed miRNAs that were revealed in the NGS results. Results There was a positive correlation between newborn body weight and pregestational BMI of pregnant individuals (r = 0.48,P < 0.001). One hundred and eight miRNAs were differentially expressed between the normal and overweight/obese groups, which target genes were enriched in the metabolic pathway. Five C19MC miRNAs (miR-516a-5p, miR-516b-5p, miR-520a-3p, miR-1323, miR-523-5p) were upregulated in the overweight/obese group. Target enrichment analysis suggests their involvement in post-embryonic development, lipid and glucose homeostasis, T cell differentiation and nervous system development. Conclusions C19MC miRNA expression upregulation in the fetal circulation during the gestation of overweight/obese pregnant women may contribute to altered multisystem metabolic pathway development in their offspring.