LYME BORRELIOSIS IN GENETICALLY RESISTANT AND SUSCEPTIBLE MICE WITH SEVERE COMBINED IMMUNODEFICIENCY
LYME BORRELIOSIS IN GENETICALLY RESISTANT AND SUSCEPTIBLE MICE WITH SEVERE COMBINED IMMUNODEFICIENCY
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DOI：
10.4269/ajtmh.1992.47.605
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发表时间：
1992-11-01
影响因子：
3.3
通讯作者：
SMITH, AL
中科院分区：
文献类型：
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作者：
BARTHOLD, SW;SIDMAN, CL;SMITH, AL
在遗传抗性C.B-17和敏感的C3 H/He（C3 H）小鼠中检查了莱姆病的演变，这些小鼠是严重联合免疫缺陷（scid）基因纯合子，或它们的免疫活性对应物。C.B-17、C.B-17-scid、C3 H和C3 H-scid小鼠皮内接种10（4）伯氏疏螺旋体，并在接种后第14、21、30、45和60天进行检查。在30天内检测到螺旋体血症，但所有组在45天内均被清除。肾脏和脑的培养结果不一致，但大多数小鼠的脾脏和耳穿孔样本均为阳性。免疫活性C.B-17和C3 H小鼠血清转化为B，IgG滴度相等。而C.B-17-scid和C3 H-scid小鼠没有血清转化。在第14天，几乎所有基因型的所有关节都发生了关节炎，C.B-17、C.B-17-scid和C3 H小鼠的关节炎严重程度相同，但C3 H-scid小鼠的关节炎更严重。到第30天和第45天，免疫活性小鼠的关节炎开始消退，C3 H小鼠比C.B-17小鼠具有更严重的疾病。关节炎在C.B-17-scid和C3 H-scid小鼠中持续存在。在第14天，所有组中心脏炎的发生程度相同，在scid小鼠中仍然活跃，但在免疫活性小鼠中在稍后的时间间隔消退。银染显示scid小鼠发炎的滑膜组织中有许多螺旋体，其他组织中也有少量螺旋体。这些研究表明，特异性免疫与关节炎发生或遗传决定的关节炎易感性无关，但与关节炎和心脏炎消退有关。
The evolution of Lyme borreliosis was examined in genetically resistant C.B-17 and susceptible C3H/He(C3H) mice homozygous for the severe combined immune deficiency (scid) gene, or their immunocompetent counterparts. The C.B-17, C.B-17-scid, C3H, and C3H-scid mice were inoculated intradermally with 10(4) Borrelia burgdorferi and examined on days 14, 21, 30, 45, and 60 after inoculation. Spirochetemia was detected through 30 days, but was cleared in all groups by 45 days. Kidney and brain were inconsistently culture positive, but spleen and ear punch samples were positive in most mice. Immunocompetent C.B-17 and C3H mice seroconverted with equivalent IgG titers to B. burgdorferi, while C.B-17-scid and C3H-scid mice did not seroconvert. Arthritis occurred in nearly all joints examined in all genotypes on day 14, was of equal severity among C.B-17, C.B-17-scid, and C3H mice, but was more severe in C3H-scid mice. By days 30 and 45, arthritis began to resolve in immunocompetent mice, with C3H mice having more severe disease than C.B-17 mice. Arthritis persisted in C.B-17-scid and C3H-scid mice. Carditis occurred to an equal degree in all groups on day 14, remained active in scid mice, but regressed in immunocompetent mice at later intervals. Many spirochetes were visualized with silver stain in inflamed synovial tissues of scid mice, and were present in other tissues in smaller numbers. These studies show that specific immunity is not involved in arthritogenesis or genetically determined susceptibility to arthritis, but is involved in arthritis and carditis regression.