PROMOTION OF TRIPLEX FORMATION BY 2′-O,4′-C-METHYLENE BRIDGED NUCLEIC ACID (2′,4′-BNA) MODIFICATION: THERMODYNAMIC AND KINETIC STUDIES
PROMOTION OF TRIPLEX FORMATION BY 2′-O,4′-C-METHYLENE BRIDGED NUCLEIC ACID (2′,4′-BNA) MODIFICATION: THERMODYNAMIC AND KINETIC STUDIES
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2′-O,4′-C-亚甲基桥核酸 (2′,4′-BNA) 修饰促进三链体形成：热力学和动力学研究
DOI：
10.1081/ncn-100002525
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
B. Hirschbein
中科院分区：
文献类型：
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作者：
J. Nelson;K. Fearon;Mark Q. Nguyen;S. Mccurdy;J. Frediani;M. Foy;B. Hirschbein
我们分析了在中性pH条件下，嘧啶基序三链体不稳定的条件下，三链体形成寡核苷酸（TFO）的2′-O，4′-C-亚甲基桥接核酸（2′，4 ′-BNA）修饰对嘧啶基序三链体形成的影响。在pH6.8条件下，2′，4 ′-BNA修饰的TFO与嘧啶基序形成三链体的结合常数比未修饰的TFO大约20倍。在中性pH下，通过2′，4 ′-BNA修饰观察到的结合常数的增加是由于解离速率常数的显著降低。
We analyzed the effect of 2′-O,4′-C-methylene bridged nucleic acid (2′,4′-BNA) modification of triplex-forming oligonucleotide (TFO) on pyrimidine motif triplex formation at neutral pH, a condition where pyrimidine motif triplexes are unstable. The binding constant of the pyrimidine motif triplex formation at pH 6.8 with 2′,4′-BNA modified TFO was about 20 times larger than that observed with unmodified TFO. The observed increase in the binding constant at neutral pH by the 2′,4′-BNA modification resulted from the considerable decrease in the dissociation rate constant.