Microarray analysis of the in vivo effects of hypophysectomy and growth hormone treatment on gene expression in the rat
Microarray analysis of the in vivo effects of hypophysectomy and growth hormone treatment on gene expression in the rat
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DOI：
10.1210/en.142.7.3163
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发表时间：
2001-07-01
期刊：
影响因子：
4.8
通讯作者：
Norstedt, G
中科院分区：
文献类型：
--
作者：
Flores-Morales, A;Ståhlberg, N;Norstedt, G
含有3000种不同大鼠基因的互补DNA微阵列被用来研究严重的激素缺乏（垂体切除术）对心脏、肝脏和肾脏基因表达模式的影响。杂交信号被认为是从大多数的阵列互补DNA，尽管如此，组织特异性表达模式可以划定。垂体切除影响了参与多种细胞功能的基因表达。作为对这种情况的反应，来自每个组织的16-29%的检测到的转录物改变了表达水平。慢性治疗垂体切除动物与人类生长激素也引起显着的变化，基因表达模式。该研究证实了以前的知识，在上述情况下，某些基因表达的变化，并提供了新的信息，垂体切除术和慢性人类生长激素的影响，在大鼠。此外，我们已经确定了几个新的基因，响应生长激素治疗。我们的研究结果代表了对激素缺乏状态下基因表达变化的更全面理解的第一步。
Complementary DNA microarrays containing 3000 different rat genes were used to study the consequences of severe hormonal deficiency (hypophysectomy) on the gene expression patterns in heart, liver, and kidney. Hybridization signals were seen from a majority of the arrayed complementary DNAs; nonetheless, tissue-specific expression patterns could be delineated. Hypophysectomy affected the expression of genes involved in a variety of cellular functions. Between 16-29% of the detected transcripts from each tissue changed expression level as a reaction to this condition. Chronic treatment of hypophysectomized animals with human GH also caused significant changes in gene expression patterns. The study confirms previous knowledge concerning certain gene expression changes in the above-mentioned situations and provides new information regarding hypophysectomy and chronic human GH effects in the rat. Furthermore, we have identified several new genes that respond to GH treatment. Our results represent a first step toward a more global understanding of gene expression changes in states of hormonal deficiency.