Bone crystal sizes: a comparison of transmission electron microscopic and X-ray diffraction line width broadening techniques.
Bone crystal sizes: a comparison of transmission electron microscopic and X-ray diffraction line width broadening techniques.
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DOI：
10.3109/03008209409061969
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发表时间：
1994
影响因子：
2.9
通讯作者：
Vivi Ziv;S. Weiner
中科院分区：
文献类型：
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作者：
Vivi Ziv;S. Weiner
骨晶体特别小，因此估计其大小已被证明是困难的，获得的值不一致。在这里，我们使用不同年龄的大鼠骨晶体，以及不同的合成碳酸磷灰石晶体，比较两种常用的方法来确定骨晶体的大小。一种方法涉及直接测量晶体的长度和宽度，而不是厚度，从分散晶体的透射电子显微镜（TEM）照片。第二种方法利用X射线衍射线宽度展宽来估计晶体的平均长度。我们的结论是线宽展宽值往往反映晶体的相干长度，而不是整个晶体的物理尺寸。TEM测量提供了平均晶体长度和宽度及其范围的可靠估计。然而，样品制备程序会导致易碎晶体的破裂，这可能导致低估体内晶体的大小。
Bone crystals are particularly small and hence estimating their sizes have proved to be difficult and values obtained inconsistent. Here we use rat bone crystals of different ages, as well as different synthetic carbonate apatite crystals, to compare two methods commonly used for determining bone crystal sizes. One method involves direct measurement of crystal lengths and widths, but not thicknesses, from transmission electron microscope (TEM) photographs of dispersed crystals. The second method utilizes X-ray diffraction line width broadening to estimate the average length of crystals. We conclude that line width broadening values tend to reflect crystal coherence lengths rather than the physical dimensions of the whole crystal. TEM measurements provide reliable estimates of average crystal lengths and widths and their ranges. Sample preparation procedures, however, cause breakage of the fragile crystals, which probably results in underestimates of in vivo crystal sizes.