Hippocampal astrocytes encode reward location
Hippocampal astrocytes encode reward location
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DOI：
10.1038/s41586-022-05146-6
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发表时间：
2022-08-31
期刊：
影响因子：
64.8
通讯作者：
Goshen, Inbal
中科院分区：
文献类型：
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作者：
Doron, Adi;Rubin, Alon;Goshen, Inbal
星形胶质细胞的钙动力学与感觉信息的编码有关(1-5)，而星形胶质细胞中钙的调节已被证明影响行为(6-10)。然而，缺乏对清醒小鼠海马区星形胶质细胞实时钙活性的纵向研究。在这里，我们使用双光子显微镜对小鼠在熟悉的或新的虚拟环境中奔跑以获得水奖励的CA1星形胶质细胞进行慢性成像。我们发现，星形胶质细胞在熟悉的环境中对奖赏位置表现出持续的斜坡活动，而在新的环境中则不是。在熟悉的环境中改变奖励地点也会导致坡度减小。经过额外的训练后，随着老鼠逐渐熟悉新的环境或新的奖励地点，斜坡重新建立起来。使用线性解码器，我们可以单从星形胶质细胞的活动来预测老鼠在熟悉的环境中的位置。我们不能在新的环境中做同样的事情，这表明星形细胞活动的空间调节是经验依赖的。我们的结果表明，星形胶质细胞可以在空间环境中编码预期的奖励位置，从而扩展了它们已知的计算能力和在认知功能中的作用。
Astrocytic calcium dynamics has been implicated in the encoding of sensory information(1-5), and modulation of calcium in astrocytes has been shown to affect behaviour(6-10). However, longitudinal investigation of the real-time calcium activity of astrocytes in the hippocampus of awake mice is lacking. Here we used two-photon microscopy to chronically image CA1 astrocytes as mice ran in familiar or new virtual environments to obtain water rewards. We found that astrocytes exhibit persistent ramping activity towards the reward location in a familiar environment, but not in a new one. Shifting the reward location within a familiar environment also resulted in diminished ramping. After additional training, as the mice became familiar with the new context or new reward location, the ramping was re-established. Using linear decoders, we could predict the location of the mouse in a familiar environment from astrocyte activity alone. We could not do the same in a new environment, suggesting that the spatial modulation of astrocytic activity is experience dependent. Our results indicate that astrocytes can encode the expected reward location in spatial contexts, thereby extending their known computational abilities and their role in cognitive functions.