Size-Dependent Cytotoxicity of Monodisperse Silica Nanoparticles in Human Endothelial Cells
Size-Dependent Cytotoxicity of Monodisperse Silica Nanoparticles in Human Endothelial Cells
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DOI：
10.1002/smll.200800461
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发表时间：
2009-04-06
期刊：
影响因子：
13.3
通讯作者：
Hoet, Peter H.
中科院分区：
文献类型：
--
作者：
Napierska, Dorota;Thomassen, Leen C. J.;Hoet, Peter H.
研究了不同尺寸的单分散无定形球形二氧化硅颗粒对内皮细胞（EAHY 926细胞系）活力的影响。结果表明，暴露于二氧化硅纳米颗粒以剂量相关的方式在EAHY 926细胞系中引起细胞毒性损伤（如乳酸脱氢酶（LDH）释放所示）和细胞存活率降低（如通过四唑还原，MTT，测定所确定）。导致细胞存活率降低50%的浓度（TC 50）的最小颗粒（14-，15-和16-nm直径）范围为33至47 μ g cm（-2）的细胞培养物与较大的纳米颗粒的评估值显著不同：89和254 μ g cm（-2）（直径分别为19和60 nm）。两个细二氧化硅颗粒的直径为104和335 nm显示非常低的细胞毒性反应相比，纳米尺寸的颗粒与TC 50值分别为1095和1087 μ g cm（-2）。较小的颗粒似乎也更快地影响暴露的细胞，在短短几个小时内观察到细胞死亡（通过坏死）。测试颗粒的表面积是确定单分散无定形二氧化硅纳米颗粒毒性的重要参数。
The effect that monodisperse amorphous spherical silica particles of different sizes have on the viability of endothelial cells (EAHY926 cell line) is investigated. The results indicate that exposure to silica nanoparticles causes cytotoxic damage (as indicated by lactate dehydrogenase (LDH) release) and a decrease in cell survival (as determined by the tetrazolium reduction, MTT, assay) in the EAHY926 cell line in a dose-related manner. Concentrations leading to a 50% reduction in cell viability (TC50) for the smallest particles tested (14-, 15-, and 16-nm diameter) ranging from, 33 to 47 mu g cm(-2) of cell culture differ significantly from values assessed for the bigger nanoparticles: 89 and 254 mu g cm(-2) (diameter of 19 and 60 mn, respectively). Two fine silica particles with diameters of 104 and 335 nm show very low cytotoxic response compared to nanometer-sized particles with TC50 values of 1095 and 1087 mu g cm(-2), respectively. The smaller particles also appear to affect the exposed cells faster with cell death (by necrosis) being observed within just a few hours. The surface area of the tested particles is an important parameter in determining the toxicity of monodisperse amorphous silica nanoparticles.