Imaging of single light-responsive clock cells reveals fluctuating free-running periods
Imaging of single light-responsive clock cells reveals fluctuating free-running periods
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DOI：
10.1038/ncb1232
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发表时间：
2005-03-01
影响因子：
21.3
通讯作者：
Whitmore, D
中科院分区：
文献类型：
--
作者：
Carr, AJF;Whitmore, D
斑马鱼的组织和细胞系含有直接对光做出反应的生物钟(1，2)。利用荧光激活的细胞分选方法，我们分离出了含有报告构建体zfperio4-荧光素酶(3)的克隆细胞系。生物发光分析表明，细胞群体内的振荡在持续的黑暗中受到抑制。然而，单细胞成像显示，单个细胞继续振荡，但在自由运行阶段具有广泛的相位分布和明显的随机波动。因为这些细胞是直接对光响应的，所以我们可以很容易地跟踪相移到单个光脉冲。在这里，我们展示了光的作用，重置去同步的细胞振荡到一个共同的阶段，以及稳定随后的自由运行周期。
Zebrafish tissues and cell lines contain circadian clocks that respond directly to light(1,2). Using fluorescence-activated cell sorting, we have isolated clonal cell lines that contain the reporter construct, zfperiod4-luciferase(3). Bioluminescent assays show that oscillations within cell populations are dampened in constant darkness. However, single-cell imaging reveals that individual cells continue to oscillate, but with widely distributed phases and marked stochastic fluctuations in free-running period. Because these cells are directly light responsive, we can easily follow phase shifts to single light pulses. Here we show that light acts to reset desynchronous cellular oscillations to a common phase, as well as stabilize the subsequent free-running period.