Facile enzymatic synthesis of sugar 1-phosphates as substrates for phosphorylases using anomeric kinases
Facile enzymatic synthesis of sugar 1-phosphates as substrates for phosphorylases using anomeric kinases
复制标题
DOI：
10.1016/j.carres.2014.10.014
复制
发表时间：
2015-01-12
影响因子：
3.1
通讯作者：
Kitaoka, Motomitsu
中科院分区：
文献类型：
--
作者：
Liu, Yuan;Nishimoto, Mamoru;Kitaoka, Motomitsu
通过丙酮酸激酶和相应的异头激酶的联合作用，以良好的产率从磷酸烯醇式酸和相应的糖以克级产生三种糖1-磷酸，它们是磷酸化酶的供体底物。通过两个电渗析步骤纯化这些糖1-磷酸。最终分离出作为二钾盐晶体的α-D-半乳糖1-磷酸。α-D-甘露糖1-磷酸和2-乙酰氨基-2-脱氧-α-D-葡萄糖1-磷酸作为双（环己基铵）盐的晶体分离。(C)2014爱思唯尔有限公司版权所有。
Three sugar 1-phosphates that are donor substrates for phosphorylases were produced at the gram scale from phosphoenolpyruvic acid and the corresponding sugars by the combined action of pyruvate kinase and the corresponding anomeric kinases in good yields. These sugar 1-phosphates were purified through two electrodialysis steps. alpha-D-Galactose 1-phosphate was finally isolated as crystals of dipotassium salts. alpha-D-Mannose 1-phosphate and 2-acetamido-2-deoxy-alpha-D-glucose 1-phosphate were isolated as crystals of bis(cyclohexylammonium) salts. (C) 2014 Elsevier Ltd. All rights reserved.