Protective effects of melatonin and N-acetylserotonin on aflatoxin B1-induced lipid peroxidation in rats
Protective effects of melatonin and N-acetylserotonin on aflatoxin B1-induced lipid peroxidation in rats
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DOI：
10.1002/cbf.1438
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发表时间：
2008-04-01
影响因子：
3.6
通讯作者：
Karbownik-Lewinska, Malgorzata
中科院分区：
文献类型：
--
作者：
Gesing, Adam;Karbownik-Lewinska, Malgorzata
黄曲霉毒素B1(AFB1)是一种具有较强肝毒性和致癌作用的真菌毒素。活性氧被认为参与了黄曲霉毒素毒性的主要机制。褪黑素(Mel)是一种具有抗氧化活性的激素。N-乙酰5-羟色胺(NAC-5HT)是MEL的直接前体。褪黑素被证明在人类和动物中是完全安全的。本研究旨在探讨MEL或NAC-5HT对黄曲霉毒素B_1(AFB1)所致雄性Wistar大鼠脂质过氧化(LPO)的潜在保护作用。MEL和NAC-5HT腹腔注射(Ip)。下午晚些时候(16：00-18：00)注射3周，每天注射20 mg kg(-1)bw。ARB1(50亩GKG(-1)BW/d)ip。在注射吲哚胺前6h。测定肝、脑、肺、睾丸、肾组织匀浆中丙二醛+4-羟基烯(MDA+4-HDA)含量。组织匀浆中的LPO水平以每毫克蛋白质中的MDA+4-HDA(NmoL)量来表示。黄曲霉毒素B_1能增加肝、肺、脑和睾丸中的LPO，但不能增加肾脏中的LPO。MEL或NAC-5HT均能完全阻止注射AFB1所致的LPO升高。褪黑素可以被认为是黄曲霉毒素1中毒的保护性药理制剂，而NAC-5HT对黄曲霉毒素诱导的LPO的保护作用扩大了人们对其抗氧化特性的认识。版权所有(C)2007 John Wiley&Sons，Ltd.
Aflatoxin B1 (AFB1) is a potent hepatotoxic and hepatocarcinogenic mycotoxin. Reactive oxygen species are considered to participate in the main mechanism of aflatoxin toxicity. Melatonin (Mel) is a hormone which has antioxidative activities. N-acetylserotonin (NAc-5HT) is an immediate precursor of Mel. Melatonin is documented to be completely safe in humans and animals. The aim of our study was to examine the potential protective effects of Mel or NAc-5HT against lipid peroxidation (LPO), caused by AFB1 in male Wistar rats. Mel and NAc-5HT were intraperitoneally (i.p.) injected for 3 weeks in late afternoon (16:00-18:00) injections (20mg kg(-1) BW/daily). ARB1 (50 mu gkg(-1) BW/daily) was administered i.p. 6h prior to indoleamine injections. Concentrations of malondialdehyde + 4-hydroxyalkenals (MDA + 4-HDA), as an index of LPO, were measured in liver, brain, lung, testis and kidney homogenates. The level of LPO in tissue homogenates was expressed as the amount of MDA + 4-HDA (nmol) per milligram of protein. AFB1 increased LPO in the liver, lung, brain and testis, but not the kidney. The increase of LPO caused by AFB1 injections was completely prevented by either Mel or NAc-5HT in all the tissues examined. Melatonin can be considered as a protective pharmacological agent in intoxication with AFB1 and the protective effect of NAc-5HT against aflatoxin-induced LPO broadens the knowledge about its antioxidative properties. Copyright (c) 2007 John Wiley & Sons, Ltd.