Intracerebroventricular injection of RFRP-3 delays puberty onset and stimulates growth hormone secretion in female rats.
Intracerebroventricular injection of RFRP-3 delays puberty onset and stimulates growth hormone secretion in female rats.
复制标题
侧脑室注射 RFRP-3 延迟雌性大鼠青春期开始并刺激生长激素分泌
DOI：
10.1186/s12958-017-0254-5
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发表时间：
2017-05-02
期刊：
影响因子：
--
通讯作者：
Yu J
中科院分区：
文献类型：
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作者：
Han X;He Y;Zeng G;Wang Y;Sun W;Liu J;Sun Y;Yu J
研究背景青春期启动是一个复杂的、有组织的、多层次调控的生物学过程，其生理病理机制尚未阐明。RFRP-3是哺乳动物促性腺激素抑制激素的直系同源物，参与抑制哺乳动物促性腺激素的合成和释放。然而，目前还不清楚是否RFRP-3参与调节青春期development.MethodsThis研究探讨下丘脑RFRP-3神经肽在青春期的开始在年轻的雌性大鼠的功能意义和调节机制。在出生后第22天，我们将套管植入雌性幼鼠的侧脑室。从出生后第28天到出生后第36天，每天进行两次脑室内注射RFRP-3或载体。为了探讨是否青春期的开始受到影响，我们检查了体重，阴道开口的年龄，血清激素水平，子宫和卵巢的发展，和下丘脑Kiss-1 mRNA expression.ResultsIntracerebroventricular注射RFRP-3显着降低血清中的黄体生成素和雌二醇的浓度，延迟子宫成熟，并推迟阴道开口的时间。这项研究表明，RFRP-3可以延迟青春期的开始在年轻的雌性大鼠; Kiss-1 mRNA的表达在RFRP-3组被有效地抑制。此外，我们的数据表明，RFRP-3升高血清生长激素levels.ConclusionsThese数据表明，侧脑室注射的RFRP-3显着延迟青春期在雌性大鼠的发病。此外，RFRP-3可能与青春期前生长激素分泌的增加有关。
BackgroundPuberty onset is a complex, organized biological process with multilevel regulation, and its physiopathological mechanisms are yet to be elucidated. RFRP-3, the mammalian ortholog to gonadotropin-inhibitory hormone, is implicated in inhibiting the synthesis and release of gonadotropin in mammals. However, it is unclear whether RFRP-3 participates in regulating pubertal development.MethodsThis study investigated the functional significance and regulatory mechanism of hypothalamic RFRP-3 neuropeptide in the onset of puberty in young female rats. On postnatal day 22, we implanted cannulas into the lateral ventricles of female rat pups. From postnatal day 28 to postnatal day 36, the intracerebroventricular injection of RFRP-3, or vehicle, was conducted twice a day. To investigate whether puberty onset was affected, we examined the body weight, age of vaginal opening, serum hormone levels, uterus and ovary development, and hypothalamic Kiss-1 mRNA expression.ResultsIntracerebroventricular injection of RFRP-3 significantly decreased the serum concentrations of luteinizing hormone and estradiol, delayed uterine maturation, and postponed the time of vaginal opening. This study suggests that RFRP-3 can delay the onset of puberty in young female rats; the expression of Kiss-1 mRNA is potently inhibited in the RFRP-3 group. Moreover, our data show that RFRP-3 elevates serum growth hormone levels.ConclusionsThese data suggest that intracerebroventricular injection of RFRP-3 significantly delays the onset of puberty in female rats. Additionally, RFRP-3 may be associated with prepubertal rise in the secretion of growth hormone.