Evaluation of TRPM (transient receptor potential melastatin) genes expressions in myocardial ischemia and reperfusion
Evaluation of TRPM (transient receptor potential melastatin) genes expressions in myocardial ischemia and reperfusion
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DOI：
10.1007/s11033-014-3139-0
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发表时间：
2014-05-01
影响因子：
2.8
通讯作者：
Bagci, Cahit
中科院分区：
文献类型：
--
作者：
Demir, Tuncer;Yumrutas, Onder;Bagci, Cahit
在本研究中，TRPM 1，TRPM 2，TRPM 3，TRPM 4，TRPM 5，TRPM 6，TRPM 7和TRPM 8基因的表达水平在缺血/再灌注（IR）后的心脏组织中进行了评估。本实验选用雄性白化病Wistar大鼠30只，随机分为3组，每组10只，第1组为对照组，第2组为缺血组（缺血60 min），第3组为缺血再灌注组（缺血60 min后再灌注48 h，再灌注48 h）。用半定量逆转录-PCR分析TRPM基因的表达水平。与缺血对照相比，TRPM 2、TRPM 4和TRPM 6的表达水平没有变化，而TRPM 7的表达水平增加。然而，TRPM 1、TRPM 3、TRPM 5和TRPM 8在心脏组织中不表达。心肌组织病理学分析显示，IR损伤最严重的是暴露于IR的心肌组织，TRPM 7的表达与IR损伤呈正相关。
In the present study, the expression levels of TRPM1, TRPM2, TRPM3, TRPM4, TRPM5, TRPM6, TRPM7, and TRPM8 genes were evaluated in heart tissues after ischemia/reperfusion (IR). For this study, 30 albino male Wistar rats were equally divided into three groups as follows: Group 1: control group (n:10), Group II: ischemia group (ischemia for 60 min) (n:10) and Group III: IR (reperfusion 48 h after ischemia for 60 min and reperfusion for 48 h). The expression levels of the TRPM genes were analyzed by semi-quantitative reverse transcriptase-PCR. When compared to the ischemia control, the expression levels of TRPM2, TRPM4, and TRPM6 did not change, whereas that of TRPM7 increased. However, TRPM1, TRPM3, TRPM5, and TRPM8 were not expressed in heart tissue. Histopathological analysis of the myocardial tissues showed that the structures that were most damaged were those exposed to IR. The findings showed that there is a positive relationship between TRPM7 expression and myocardial IR injury.