In Vivo Cellular Infiltration and Remodeling in a Decellularized Ovine Osteochondral Allograft.
In Vivo Cellular Infiltration and Remodeling in a Decellularized Ovine Osteochondral Allograft.
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DOI：
10.1089/ten.tea.2016.0149
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发表时间：
2016-11
影响因子：
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通讯作者：
T. Novak;Kateri Fites Gilliland;Xin-xiang Xu;L. Worke;A. Ciesielski;G. Breur;C. Neu
中科院分区：
文献类型：
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作者：
T. Novak;Kateri Fites Gilliland;Xin-xiang Xu;L. Worke;A. Ciesielski;G. Breur;C. Neu
作为退行性疾病和创伤事件的潜在解决方案，脱细胞组织取代了缺失组织的部位，并为组织向内生长和再生提供了相关的生物化学和微观结构，人们对脱细胞组织的兴趣不断增加。骨关节炎是一种进行性的、使人衰弱的疾病，通常是由原本光滑的关节软骨表面形成局灶性缺损引起的。脱细胞软骨组织保持了天然细胞外基质的结构复杂性，有可能为局部缺损或大组织损伤提供临床相关的解决方案，甚至可能规避或补充现有技术，例如微骨折和镶嵌成形术。然而，目前尚不清楚体内脱细胞软骨的植入是否可以提供机械和生物化学相关的平台来促进细胞重塑和修复。我们检查了植入绵羊滑车沟中的整个脱细胞同种异体骨软骨移植物，以研究与空缺损和对侧对照（健康软骨）相比的细胞重塑和修复组织质量。术后 3 个月，在脱细胞组织和空缺损中均观察到细胞，尽管两者的水平均显着低于健康软骨。定性和定量组织学分析表明，脱细胞植入物的软骨特征与健康软骨组相似。定量磁共振成像无创分析显示所有组之间的 T1ρ 和 T2* 没有差异。对修复组织机械性能的研究表明，与健康软骨相比，脱细胞植入物和空缺损的弹性明显较低，但摩擦学量相似。总体而言，这项研究表明，脱细胞软骨植入物在体内环境中会发生细胞重塑，并可能为软骨缺损干预提供潜在的组织工程解决方案。
Interest in decellularized tissues has steadily gained as potential solutions for degenerative diseases and traumatic events, replacing sites of missing tissue, and providing the relevant biochemistry and microstructure for tissue ingrowth and regeneration. Osteoarthritis, a progressive and debilitating disease, is often initiated with the formation of a focal defect in the otherwise smooth surface of articular cartilage. Decellularized cartilage tissue, which maintains the structural complexity of the native extracellular matrix, has the potential to provide a clinically relevant solution to focal defects or large tissue damage, possibly even circumventing or complementing current techniques such as microfracture and mosaicplasty. However, it is currently unclear whether implantation of decellularized cartilage in vivo may provide a mechanically and biochemically relevant platform to promote cell remodeling and repair. We examined whole decellularized osteochondral allografts implanted in the ovine trochlear groove to investigate cellular remodeling and repair tissue quality compared to empty defects and contralateral controls (healthy cartilage). At 3 months postsurgery, cells were observed in both the decellularized tissue and empty defects, although both at significantly lower levels than healthy cartilage. Qualitative and quantitative histological analysis demonstrated maintenance of cartilage features of the decellularized implant similar to healthy cartilage groups. Noninvasive analysis by quantitative magnetic resonance imaging showed no difference in T1ρ and T2* between all groups. Investigation of the mechanical properties of repair tissue showed significantly lower elasticity in decellularized implants and empty defects compared to healthy cartilage, but similar tribological quantities. Overall, this study suggests that decellularized cartilage implants are subject to cellular remodeling in an in vivo environment and may provide a potential tissue engineering solution to cartilage defect interventions.