Proliferation inhibition of cisplatin and aquaporin 5 expression in human ovarian cancer cell CAOV3
Proliferation inhibition of cisplatin and aquaporin 5 expression in human ovarian cancer cell CAOV3
复制标题
人卵巢癌细胞CAOV3中顺铂和水通道蛋白5表达的增殖抑制
DOI：
10.1007/s00404-011-1908-8
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发表时间：
2012-01-01
影响因子：
2.6
通讯作者：
Chen, Xuejun
中科院分区：
文献类型：
--
作者：
Yang, Jianhua;Yan, Chunxiao;Chen, Xuejun
目的探讨顺铂对卵巢癌细胞株CAOV 3增殖的抑制作用及其与水通道蛋白5（aquaporin 5，AQP 5）表达的关系。方法采用MTT法检测顺铂或NF-κB抑制剂PDTC对CAOV 3细胞增殖的影响。Western blot和RT-PCR检测AQP 5和NF-κB p65的表达。结果顺铂可诱导CAOV 3细胞AQP 5、NF-κB和IκBα的表达降低，且呈浓度依赖性，且与细胞生长速度呈正相关（r= 0.607，P < 0.05）。当顺铂浓度为10 μg/ml时，AQP 5、NF-κB p65和IκBα在6-12 h迅速升高，24 h下降，至72 h仍维持在低水平。PDTC可诱导AQP 5蛋白表达下降，且AQP 5蛋白表达与NF-κB p65、IκBα呈正相关（r= 0.894，0.857;P< 0.05）。AQP 5可能成为卵巢癌治疗的潜在靶点。
AimsThe purpose of this study is to investigate the association between proliferation inhibition of cisplatin and aquaporin 5 (AQP5) expression and its regulation in ovarian carcinoma cell CAOV3.MethodsCell growth rate was measured by MTT after CAOV3 cells were incubated with cisplatin or NF-κB inhibitor PDTC. Western blot and RT-PCR were used to detect the expression of AQP5 and NF-κB p65.ResultsOur results showed that expression of AQP5, NF-κB in cytoplasm and karyon and IκBα in cytoplasm protein in CAOV3 cells can be induced to decrease by cisplatin with concentration-dependent manner, and there is a positive correlation between AQP5 protein and cell growth rate (r= 0.607,P< 0.05). When cells were incubated with 10 μg/ml cisplatin, AQP5, NF-κB p65, and IκBα increased rapidly at 6–12 h, but decreased at 24 h, remain on low level until to 72 h. Expression of AQP5 could be induced to decrease by PDTC, and a positive correlation between AQP5 protein expression and NF-κB p65 and IκBα (r= 0.894, 0.857;P< 0.05).ConclusionsProliferation inhibition of cisplatin is related with AQP5 expression, and NF-κB may be involved in mechanism of AQP5 regulation. AQP5 will be potential target for therapy of ovarian carcinoma.