Transforming Growth Factor‐β and Hepatocyte Growth Factor Produced by Gastric Fibroblasts Stimulate the Invasiveness of Scirrhous Gastric Cancer Cells
Transforming Growth Factor‐β and Hepatocyte Growth Factor Produced by Gastric Fibroblasts Stimulate the Invasiveness of Scirrhous Gastric Cancer Cells
复制标题
胃成纤维细胞产生的转化生长因子-β和肝细胞生长因子刺激硬质胃癌细胞的侵袭性
DOI：
--
复制
发表时间：
1997
期刊：
影响因子：
--
通讯作者：
M. Sowa
中科院分区：
文献类型：
--
作者：
Tohru Inoue;Yong‐suk Chung;M. Yashiro;S. Nishimura;T. Hasuma;S. Otani;M. Sowa
硬质胃癌的特点是癌细胞在间质中快速浸润，成纤维细胞广泛生长。在本研究中，我们使用侵袭试验检查了胃成纤维细胞对硬质胃癌细胞系 OCUM-2D 侵袭性的影响。胃成纤维细胞来源的条件培养基（CM）显着刺激 OCUM-2D 细胞的侵袭性，转化生长因子-β（TGF-β）和肝细胞生长因子（HGF）也是如此。抗TGF-β中和抗体或抗HGF中和抗体显着抑制胃成纤维细胞来源的CM的刺激活性。在胃成纤维细胞来源的 CM 中检测到 TGF-β 和 HGF，在 OCUM-2D 细胞上表达 TGF-β 受体和 C-met（HGF 受体）。因此，胃成纤维细胞产生的TGF-β和HGF似乎影响硬质胃癌细胞的侵袭性。在 OCUM-2D 细胞衍生的条件培养基中也检测到了 TGF-β，但未检测到 HGF。 TGF-β 似乎以旁分泌和自分泌方式影响 OCUM-2D 细胞的侵袭性。
Scirrhous gastric carcinoma is characterized by cancer cells that infiltrate rapidly in the stroma with extensive growth of fibroblasts. In the present study, we examined the effect of gastric fibroblasts on the invasiveness of a Scirrhous gastric cancer cell line, OCUM‐2D, using an invasion assay. Gastric fibroblast‐derived conditioned medium (CM) significantly stimulated the invasiveness of OCUM‐2D cells, as did transforming growth factor‐β (TGF‐β) and hepatocyte growth factor (HGF). The stimulating activity of gastric fibroblast‐derived CM was inhibited significantly by anti‐TGF‐β neutralizing antibody or anti‐HGF neutralizing antibody. TGF‐β and HGF were detected in the gastric fibroblast‐derived CM, and TGF‐β receptor and C‐met (HGF receptor) were expressed on OCUM‐2D cells. Thus, TGF‐β and HGF produced by gastric fibroblasts appear to affect the invasiveness of scirrhous gastric cancer cells. TGF‐β was also detected in the conditioned medium derived from OCUM‐2D cells, though HGF was not. TGF‐β appears to affect the invasiveness of OCUM‐2D cells in both paracrine and autocrine fashions.
DOI：
10.1073/pnas.91.11.4731
发表时间：
1994-05-24
影响因子：
11.1
作者：
RONG, S;SEGAL, S;VANDEWOUDE, GF
通讯作者：
VANDEWOUDE, GF
DOI：
10.1073/pnas.87.19.7678
发表时间：
1990-10-01
影响因子：
11.1
作者：
WELCH, DR;FABRA, A;NAKAJIMA, M
通讯作者：
NAKAJIMA, M