Staining of Dead Neurons by the Golgi Method in Autopsy Material
Staining of Dead Neurons by the Golgi Method in Autopsy Material
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DOI：
10.1007/978-1-4939-2152-2_13
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发表时间：
2015-01-01
期刊：
影响因子：
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通讯作者：
Baloyannis, Stavros J.
中科院分区：
文献类型：
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作者：
Baloyannis, Stavros J.
高尔基体银浸渍技术仍然是理想的方法，用于在福尔马林固定的脑和石蜡切片中将神经元作为一个整体进行可视化，使得能够洞察树突状乔木的形态和形态测量特征，以及对棘的形态和棘密度的估计，因为它们以独特的清晰度和精确度描绘神经细胞的轮廓。此外，高尔基体技术使在正常条件下的神经元和神经元回路之间的拓扑关系的研究，以及以下的时空形态发生的变化在退行性过程中。高尔基技术已经经历了许多修改，以增强和获得最佳和最大的可视化的神经元和神经元的过程，最小的沉淀，缩短所需的时间的程序，使准确的研究和描述的特定结构的大脑。在神经元变性和死亡的连续阶段的可视化中，高尔基体方法在变性轴突和树突、突触“终扣”、轴突终末和细胞器的可视化中起着突出的作用。此外，新版本的技术提高了精确观察中枢神经系统衰弱性疾病尸检材料中神经元变性、星形胶质细胞增殖、小胶质细胞活化和毛细血管形态的能力
Golgi silver impregnation techniques remain ideal methods for the visualization of the neurons as a whole in formalin fixed brains and paraffin sections, enabling to obtain insight into the morphological and morphometric characters of the dendritic arbor, and the estimation of the morphology of the spines and the spinal density, since they delineate the profile of nerve cells with unique clarity and precision. In addition, the Golgi technique enables the study of the topographic relationships between neurons and neuronal circuits in normal conditions, and the following of the spatiotemporal morphological alterations occurring during degenerative processes. The Golgi technique has undergone many modifications in order to be enhanced and to obtain the optimal and maximal visualization of neurons and neuronal processes, the minimal precipitations, the abbreviation of the time required for the procedure, enabling the accurate study and description of specific structures of the brain. In the visualization of the sequential stages of the neuronal degeneration and death, the Golgi method plays a prominent role in the visualization of degenerating axons and dendrites, synaptic "boutons," and axonal terminals and organelles of the cell body. In addition, new versions of the techniques increases the capacity of precise observation of the neurofibrillary degeneration, the proliferation of astrocytes, the activation of the microglia, and the morphology of capillaries in autopsy material of debilitating diseases of the central nervous system