Changes in liver PPARα mRNA expression in response to two levels of high-safflower-oil diets correlate with changes in adiposity and serum leptin in rats and mice
Changes in liver PPARα mRNA expression in response to two levels of high-safflower-oil diets correlate with changes in adiposity and serum leptin in rats and mice
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DOI：
10.1016/j.jnutbio.2006.03.003
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发表时间：
2007-02-01
影响因子：
5.6
通讯作者：
Huang, Ching-jang
中科院分区：
文献类型：
--
作者：
Hsu, Shan-Ching;Huang, Ching-jang
已知配体依赖性转录因子过氧化物酶体增殖物激活受体α（PPARα）被常见脂肪酸激活并调节各种脂质氧化途径和转运的基因表达。高脂肪饮食提供更多的脂肪酸，这可能可以通过上调 PPAR α 信号传导来增强脂质分解代谢。然而，大量摄入脂肪也可能导致肥胖。为了检查高脂肪喂养和肥胖中的 PPAR α 信号传导，本研究检查了喂食两种水平（20% 或 30% wt/wt）高红花油（SFO；富含油酸）饮食的 Wistar 大鼠和 C57BL/6J 小鼠中 PPAR α 及其一些靶基因的肝脏 mRNA 表达，直到动物表现出明显高于喂食红花油的对照组的体重（大鼠 13 周，小鼠 22 周）。 5% SFO 饮食。在各自的喂养期结束时，两组高脂饮食中仅喂养30％SFO的大鼠和喂养20％SFO的小鼠表现出显着较高的体重、白色脂肪组织重量、血清瘦素和PPARα的mRNA表达（P
The ligand-dependent transcription factor peroxisome proliferator-activated receptor alpha (PPAR alpha) is known to be activated by common fatty acids and to regulate the expression of genes of various lipid oxidation pathways and transport. High-fat diets provide more fatty acids, which presumably could enhance lipid catabolism through up-regulation of PPAR alpha signaling. However, high intake of fat could also lead to obesity. To examine PPAR alpha signaling in high-fat feeding and obesity, this study examined the hepatic mRNA expression of PPAR alpha and some of its target genes in Wistar rats and C57BL/6J mice fed two levels (20% or 30% wt/wt) of high-safflower-oil (SFO; oleic-acid-rich) diets until animals showed significantly higher body weight (13 weeks for rats and 22 weeks for mice) than those of control groups fed a 5% SFO diet. At the end of these respective feeding periods, only the rats fed 30% SFO and the mice fed 20% SFO among the two groups fed high-fat diets showed significantly higher body weight, white adipose tissue weight, serum leptin and MRNA expression of PPAR alpha (P