Changes in luminal flow rate modulate basal and bradykinin-stimulated cell [Ca2+] in aortic endothelium.
Changes in luminal flow rate modulate basal and bradykinin-stimulated cell [Ca2+] in aortic endothelium.
复制标题
管腔流速的变化调节主动脉内皮细胞的基础细胞和缓激肽刺激细胞 [Ca2+]。
DOI：
10.1002/jcp.1041510107
复制
发表时间：
1992
影响因子：
5.6
通讯作者：
ChaseJr,HS
中科院分区：
文献类型：
--
作者：
Oliver,JA;ChaseJr,HS
Hemodynamic forces influence many endothelial cell functions. The coupling between hemodynamic forces and cell function could be mediated by mechano‐sensitive ion channels present in the plasma membrane of endothelial cells. Because one of these channels is permeable to Ca2+, we tested whether hemodynamic forces influence endothelial cell Ca2+([Ca2+]i). Bovine aortic endothelial cells were grown inside cylindrical glass tubes, loaded with fura‐2, and perfused at different pressures and flow rates on the stage of a fluorescence microscope. Decreasing flow from 110 to 2 ml.min−1raised [Ca2+]ifrom 57 ± 11 to 186 ± 29 nM (mean ± SEM, p < 0.01) by increasing the entry of extracellular Ca2+into the cytoplasm. Increasing flow from 2 to 110 ml.min−1transiently decreased [Ca2+]ifrom 62 ± 3 to 33 ± 5 nM (p < 0.01) apparently due to reduced Ca2+entry and concomitant extrusion by the plasma membrane Ca2+‐ATPase. The rise in [Ca2+]iinduced by bradykinin was magnified during a decrease in flow; in control cells, 10−7M bradykinin increased [Ca2+]iby 162 ± 26 nM, whereas [Ca2+]iincreased 350 ± 67 nM (p < 0.05) in cells previously exposed to 110 ml.min−1. These observations suggest that flow‐induced changes in [Ca2+]imight be a signal‐transduction mechanism for endothelial functions responsive to hemodynamic forces and may also modulate the magnitude of hormonally mediated increases in [Ca2+]i. © 1992 Wiley‐Liss, Inc.
