Peritumoral CD16b positive-neutrophil accumulation strongly correlates with regional lymph node metastasis in thoracic esophageal squamous cell cancer
Peritumoral CD16b positive-neutrophil accumulation strongly correlates with regional lymph node metastasis in thoracic esophageal squamous cell cancer
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DOI：
10.1016/j.surg.2021.11.022
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发表时间：
2022-05-25
期刊：
影响因子：
3.8
通讯作者：
Minamiya, Yoshihiro
中科院分区：
文献类型：
--
作者：
Fujita, Hiromu;Motoyama, Satoru;Minamiya, Yoshihiro
背景：癌细胞从肿瘤转移到区域淋巴结的机制尚未完全清楚。我们假设肿瘤周围中性粒细胞积聚促进胸段食管鳞状细胞癌区域淋巴结转移。方法：2010年至2019年，126例胸段食管鳞状细胞癌患者在我院接受了根治性（R0）食管切除术，未进行术前治疗。使用石蜡包埋切除的肿瘤，我们进行了免疫组化分析的CD16 b阳性中性粒细胞聚集在瘤周区域，这是定义为一个1毫米的区域为中心的边界上分离的恶性细胞巢从宿主组织。评价瘤周CD16b染色密度与病理淋巴结转移及5年生存率的关系。虽然CD16b高和CD16b低患者的临床病理特征没有差异，病理性大淋巴结转移CD16b高表达的患者中，肿瘤侵犯淋巴管的比例（P <0.001）和5年生存率（P = 0.010）较低。此外，CD16b阳性中性粒细胞密度在肿瘤周围区域通常高于肿瘤本身。单因素和多因素分析显示，CD16b阳性中性粒细胞聚集是淋巴结转移的独立因素，比值比>25（P < .001）。结论：CD16b阳性中性粒细胞在胸段食管鳞癌中的聚集是与淋巴结转移密切相关的独立因素。(C)2021作者爱思唯尔公司出版
Background: The mechanism underlying cancer cell metastasis from the tumor to regional lymph nodes is not yet fully understood. We hypothesized that peritumoral neutrophil accumulation promotes regional lymph node metastasis in thoracic esophageal squamous cell cancer.Methods: Between 2010 and 2019, 126 thoracic esophageal squamous cell cancer patients received curative (R0) esophagectomy without preoperative treatment in our hospital. Using paraffin-embedded resected tumors, we performed immunohistochemical analysis of CD16b-positive neutrophil accumulation in the peritumoral area, which was defined as a 1-mm region centered on the border separating the malignant cell nests from the host tissue. The relationship between the density of peritumoral CD16b staining and pathological lymph node metastasis or 5-year overall survival was evaluated.Results: Although the clinicopathological characteristics of CD16b-high and CD16b-low patients did not differ, greater pathological lymph node metastasis (P < .001) and lymphatic invasion by the tumor (P - .024) and a poorer 5-year survival (P = .010) were seen in CD16b-high patients. Moreover, CD16b-positive neutrophil density was generally higher in the peritumoral area than within the tumor itself. Univariate and multivariate analyses showed that CD16b-positive neutrophil accumulation was an independent factor for lymph node metastasis with an odds ratio >25 (P < .001). On the other hand, blood neutrophil counts did not correlate with lymph node metastasis.Conclusion: Peritumoral accumulation of CD16b-positive neutrophils is an independent factor strongly correlated with lymph node metastasis in thoracic esophageal squamous cell cancer. (C) 2021 The Authors. Published by Elsevier Inc.