4 SMALL DROSOPHILA HEAT-SHOCK PROTEINS ARE RELATED TO EACH OTHER AND TO MAMMALIAN ALPHA-CRYSTALLIN
4 SMALL DROSOPHILA HEAT-SHOCK PROTEINS ARE RELATED TO EACH OTHER AND TO MAMMALIAN ALPHA-CRYSTALLIN
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DOI：
10.1073/pnas.79.7.2360
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发表时间：
1982-01-01
期刊：
影响因子：
--
通讯作者：
CRAIG, EA
中科院分区：
文献类型：
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作者：
INGOLIA, TD;CRAIG, EA
对D.确定了存在于细胞学位点67 B的黑腹菌。每个基因都有一个足够大的开放阅读框来编码一个小的热休克蛋白。预测的蛋白质的分子量与从凝胶电泳获得的实验测定值吻合良好。预测的4个小的热休克基因的氨基酸序列显示惊人的同源性超过。一半的长度。该广泛同源的区域从总共约85个氨基酸延伸至约195个氨基酸。200个氨基酸预测序列与其它蛋白质的已知序列的比较揭示了该同源区与哺乳动物α-谷氨酰胺的相应区之间的显著相似性。晶体蛋白这种结构的相似性可能的功能意义进行了讨论。
The primary base sequence of the protein coding regions of the 4 small heat shock genes of D. melanogaster present at cytological locus 67B was determined. A single open reading frame large enough to encode a small heat shock protein is found for each gene. The MW of the predicted proteins are in good agreement with experimentally determined values obtained from gel electrophoresis. The predicted amino acid sequences of the 4 small heat shock genes show striking homologies over .apprxeq. 50% of their lengths. This region of extensive homology extends from about amino acid 85 to amino acid 195 out of a total of .apprxeq. 200 amino acids. Comparison of the predicted sequence with the known sequences of other proteins revealed a remarkable similarity between this region of homology and the corresponding region of mammalian .alpha.-crystallin. The possible functional significance of this structural similarity is discussed.