Impact of Dietary Carbohydrate/Protein Ratio on Hepatic Metabolism in Land-Locked Atlantic Salmon (Salmo salar L.)
Impact of Dietary Carbohydrate/Protein Ratio on Hepatic Metabolism in Land-Locked Atlantic Salmon (Salmo salar L.)
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DOI：
10.3389/fphys.2018.01751
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发表时间：
2018-12-06
影响因子：
4
通讯作者：
Panserat, Stephane
中科院分区：
文献类型：
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作者：
Betancor, Monica B.;Olsen, Rolf E.;Panserat, Stephane
进行了普通花园实验以比较两种遗传上不同的大西洋鲑鱼（萨尔莫salar）品系，其饲喂具有高（CHO）或低（NoCHO）可消化碳水化合物（淀粉）的饮食。将来自商业养殖品系（F）或内陆种群（G）的20条鲑鱼置于两个罐中（每个罐中每个种群10条鱼）并饲喂CHO或NoCHO饲料。在实验结束时，将鱼禁食8小时，安乐死并收集血液和肝脏。饮食和人口对循环葡萄糖水平有影响，G显示低血糖，饮食淀粉增加了这一参数。相比之下，G显示出增加的血浆三酰甘油水平，无论饮食治疗，这表明葡萄糖更快地转化为三酰甘油。菌株之间代谢膳食淀粉的这种不同能力也反映在分子（基因）水平上，因为评价的大多数代谢途径主要受因子群体而不是饮食的影响。这些数据是有希望的，并建议不同的监管能力对淀粉利用内陆鲑鱼和养殖股票之间。为了充分说明内陆鲑鱼利用食物碳水化合物的能力，需要进行进一步的分析。
A common-garden experiment was carried out to compare two genetically distinct strains of Atlantic salmon (Salmo salar) fed diets with either high (CHO) or low (NoCHO) digestible carbohydrate (starch). Twenty salmon from either a commercial farmed strain (F) or a land-locked population (G) were placed in two tanks (10 fish of each population in each tank) and fed either CHO or NoCHO feeds. At the end of the experiment fish were fasted for 8 h, euthanized and blood and liver collected. Both diet and population had an effect on circulating glucose levels with G showing hypoglycaemia and dietary starch increasing this parameter. In contrast, G showed increased plasma triacylglycerol levels regardless of dietary treatment suggesting faster conversion of glucose to triacylglycerol. This different ability to metabolize dietary starch among strains was also reflected at a molecular (gene) level as most of the metabolic pathways evaluated were mainly affected by the factor population rather than by diet. The data are promising and suggest different regulatory capacities toward starch utilization between land-locked salmon and the farmed stock. Further analyses are necessary in order to fully characterize the capacity of land-locked salmon to utilize dietary carbohydrate.