Beta amyloid and hyperphosphorylated tau deposits in the pancreas in type 2 diabetes.
Beta amyloid and hyperphosphorylated tau deposits in the pancreas in type 2 diabetes.
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DOI：
10.1016/j.neurobiolaging.2008.08.019
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发表时间：
2010-09
影响因子：
4.2
通讯作者：
McGeer, Patrick L.
中科院分区：
文献类型：
--
作者：
Miklossy, Judith;Qing, Hong;Radenovic, Aleksandra;Kis, Andras;Vileno, Bertrand;Laszlo, Forro;Miller, Lisa;Martins, Ralph N.;Waeber, Gerard;Mooser, Vincent;Bosman, Fred;Khalili, Kamel;Darbinian, Nune;McGeer, Patrick L.
关键词：
强有力的流行病学证据表明阿尔茨海默病（AD）和2型糖尿病之间存在关联。为了确定淀粉样蛋白β（A β）和过度磷酸化tau蛋白是否发生在2型糖尿病中，分析了21例尸检病例（10例2型糖尿病和11例对照）的胰腺组织。通过RT-PCR在人胰腺和培养的胰岛素瘤β细胞（INS-1）中鉴定APP和tau mRNA。在胰腺提取物中通过Western印迹检测到突出的APP和tau条带。2型糖尿病患者胰岛中检测到聚集的A β、过度磷酸化的tau蛋白、泛素、载脂蛋白E、载脂蛋白（a）、IB1/JIP-1和JNK1。胰岛淀粉样沉积物中A β与胰淀素共定位。原位β折叠形成胰岛淀粉样蛋白沉积物显示红外显微光谱（SIRMS）。LPS也增加非神经元细胞中的APP。我们得出结论，A β沉积和过度磷酸化tau蛋白也与2型糖尿病相关，突出了神经退行性疾病（包括AD和2型糖尿病）的常见发病特征，并表明A β沉积和过度磷酸化tau蛋白也可能发生在大脑以外的其他器官中。
Strong epidemiologic evidence suggests an association between Alzheimer disease (AD) and type 2 diabetes. To determine if amyloid beta (Aβ) and hyperphosphorylated tau occurs in type 2 diabetes, pancreas tissues from 21 autopsy cases (10 type 2 diabetes and 11 controls) were analyzed. APP and tau mRNAs were identified in human pancreas and in cultured insulinoma beta cells (INS-1) by RT-PCR. Prominent APP and tau bands were detected by Western blotting in pancreatic extracts. Aggregated Aβ, hyperphosphorylated tau, ubiquitin, apolipoprotein E, apolipoprotein(a), IB1/JIP-1 and JNK1 were detected in Langerhans islets in type 2 diabetic patients. Aβ was co-localized with amylin in islet amyloid deposits. In situ beta sheet formation of islet amyloid deposits was shown by infrared microspectroscopy (SIRMS). LPS increased APP in non-neuronal cells as well. We conclude that Aβ deposits and hyperphosphorylated tau are also associated with type 2 diabetes, highlighting common pathogenetic features in neurodegenerative disorders, including AD and type 2 diabetes and suggesting that Aβ deposits and hyperphosphorylated tau may also occur in other organs than the brain.
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影响因子：
64.8
作者：
JOACHIM, CL;MORI, H;SELKOE, DJ
通讯作者：
SELKOE, DJ
影响因子：
16.2
作者：
GOEDERT, M;SPILLANTINI, MG;CROWTHER, RA
通讯作者：
CROWTHER, RA
DOI：
10.1073/pnas.85.20.7763
发表时间：
1988-10-01
影响因子：
11.1
作者：
COOPER, GJS;LEIGHTON, B;REID, KBM
通讯作者：
REID, KBM
影响因子：
5
作者：
Askanas, Valerie;Engel, W King
通讯作者：
Engel, W King
DOI：
10.1073/pnas.84.23.8628
发表时间：
1987-12-01
影响因子：
11.1
作者：
COOPER, GJS;WILLIS, AC;REID, KBM
通讯作者：
REID, KBM