Epstein-Baff virus encoded BALF1 gene is transcribed in Burkitt's lymphoma cell lines and in nasopharyngeal carcinoma's biopsies
Epstein-Baff virus encoded BALF1 gene is transcribed in Burkitt's lymphoma cell lines and in nasopharyngeal carcinoma's biopsies
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DOI：
10.1016/j.jcv.2004.12.016
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发表时间：
2005-09-01
影响因子：
8.8
通讯作者：
Ooka, T
中科院分区：
文献类型：
--
作者：
Cabras, G;Decaussin, G;Ooka, T
背景：EB病毒（EBV）编码两种抗凋亡细胞Bcl 2同源物，BALF 1和BHRF 1。BHRF 1具有抗凋亡活性，但在鼻咽癌（NPC）中很少表达。目的：本研究的目的是鉴定BALF 1基因。第一，在EBV阳性的B细胞系、EBV阳性的Burkitt淋巴瘤细胞系和鼻咽癌活检组织中寻找其转录表达。第二，血清依赖性抗凋亡活性检测。研究设计：首先采用逆转录酶DNA聚合酶链反应（RT-PCR）方法检测BALF 1的转录表达。结果：BALF 1在IgG处理的AKATA和TPA SB处理的P3 HR 1-TK阴性细胞系中，BALF 1在潜伏期即有表达，在裂解期表达增加更明显。由于该基因的表达不受病毒DNA合成抑制剂的影响，因此不属于晚期基因家族。当分析其在伯基特淋巴瘤（BL）衍生的细胞系和NPC活检中的转录时，所有BL衍生的细胞系和超过80%的NPC活检都转录了该基因。BALF 1转染的NIH 3 T3细胞的血清依赖性研究表明：在10%的血清中，BALF 1转染细胞的生长密度显著高于单独载体转染的NIH 3 T3细胞系，在1%的血清中，BALF 1转染细胞能够生长，但与10%的血清相比，其生长速率降低约40%，结论：BALF 1基因在EBV相关肿瘤细胞中有转录。BALF 1可使细胞不依赖于血清。这些结果提示BALF 1基因可能在EBV的致癌过程中发挥作用。(c)2005 Elsevier B. V.保留所有权利。
Background: The Epstein-Barr virus (EBV) encodes two anti-apoptotic cellular Bcl2 homologs, BALF1 and BHRF1. BHRF1 has an antiapoptotic activity but is rarely expressed in nasopharyngeal carcinoma (NPC). However, BALF1 is not yet well characterized.Objectives: The objective of the study was to characterize BALF1 gene. First, the search of its transcriptional expression in EBV-positive B cell lines, EBV-positive Burkitt's lymphoma's cell lines and nasopharyngeal carcinoma's biopsies. Second, the examination of its anti-apoptotic activity in serum dependent assays.Study design: We first analysed the transcriptional expression of BALF1 by reverse transcriptase DNA polymerase chain reaction (RT-PCR) method. For the analysis of its anti-apoptotic activity, we transfected NIH3T3 cells with pBABE-BALF1 expression plasmid and studied serum dependence of these transfectants.Results: BALF1 expression was detected in the latent stage and increased more significantly during the lytic phase in IgG-treated AKATA and TPA-SB-treated P3HR1-TK negative cell lines. As its expression was not affected by the inhibitor of viral DNA synthesis, this gene does not belong to late gene family. When analysed its transcription in Burkitt's lymphoma (BL)-derived cell lines and NPC biopsies, all BL-derived cell lines and more than 80% of NPC biopsies transcribed this gene. The study of serum dependence of BALF1-transfected NIH3T3 cells showed: with 10% of serum, BALF1 transfectants grew significantly more higher cell density than vector alone transfected NIH3T3 cell lines and with 1% of serum, BALF1 transfectants were capable of growing, but with about 40% reduced rate in comparison with those with 10% serum, while vector alone transfected NIH3T3 cells could not almost grow.Conclusion: BALF1 gene was transcribed in EBV-associated tumor cells. BALF1 could render cells to serum independent. These results suggest that BALF1 gene could play its role in EBV oncogenesis. (c) 2005 Elsevier B.V. All rights reserved.