Functional complementation in mouse-human radiation hybrids assigns the putative murine scid gene to the pericentric region of human chromosome 8.
Functional complementation in mouse-human radiation hybrids assigns the putative murine scid gene to the pericentric region of human chromosome 8.
复制标题
小鼠-人类放射杂交中的功能互补将假定的小鼠 scid 基因分配到人类 8 号染色体的中心周区域。
作者：
K. Komatsu;T. Ohta;Y. Jinno;N. Niikawa;Y. Okumura
通过 X 射线照射和体细胞融合，将人类 8 号染色体的片段引入小鼠 scid 小鼠的细胞中。由此产生的杂交克隆含有人类 DNA 片段，补充了 scid 细胞的超放射敏感性。来自这些杂交体的 Alu-PCR 产物使用染色体原位抑制杂交技术进行染色体涂色，从而将人类 HYRC（鼠科 SCID 突变的超放射敏感性，互补）基因（V(D)J 重组酶基因的候选基因）分配到人类染色体 8q11。
Fragments of human chromosome 8 were introduced into cells derived from murine scid mice via X-irradiation and somatic cell fusion. The resulting hybrid clones contained human DNA fragment(s) which complemented the hyper-radiosensitivity of the scid cells. Alu-PCR products from these hybrids were used for chromosome painting using the technique of chromosome in situ suppression hybridization, allowing assignment of the human HYRC (hyper-radiosensitivity of murine scid mutation, complementing) gene, a candidate for a V(D)J recombinase gene, to human chromosome 8q11.