Use of the Capripoxvirus homologue of Vaccinia virus 30 kDa RNA polymerase subunit (RPO30) gene as a novel diagnostic and genotyping target: Development of a classical PCR method to differentiate Goat poxvirus from Sheep poxvirus
Use of the Capripoxvirus homologue of Vaccinia virus 30 kDa RNA polymerase subunit (RPO30) gene as a novel diagnostic and genotyping target: Development of a classical PCR method to differentiate Goat poxvirus from Sheep poxvirus
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DOI：
10.1016/j.vetmic.2010.09.038
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发表时间：
2011-04-21
影响因子：
3.3
通讯作者：
Diallo, Adama
中科院分区：
文献类型：
--
作者：
Lamien, Charles Euloge;Le Goff, Christian;Diallo, Adama
绵羊痘病毒(SPPV)、山羊痘病毒(GTPV)和块状皮肤病病毒(LSDV)分别是引起绵羊、山羊和牛严重痘病毒病的主要病原。CaPVs的血清学分化是不可能的，毒株鉴定依赖于被隐含接受的假设，即病毒表现出明确的宿主特异性。然而，现在已知可能会发生交叉感染，基于首次分离该毒株的宿主动物物种的身份验证是无效的，应该用分子技术来取代，以便明确地区分毒株。为了确定用于毒株基因分型的诊断靶标，分析了编码30 kDa依赖DNA的RNA聚合酶亚基RPO30的痘苗病毒E4L基因的CaPV同源物。来自不同宿主和不同地理来源的46个菌株被纳入其中。根据宿主来源，大多数CaPV可归入三个不同的组之一：SPPV、GTPV和LSDV组。在所有SPPV分离株中都发现了一个独特的21核苷酸缺失，这被用来开发一种基于RPO30的经典PCR测试来区分SPPV和GTPV，这将使快速鉴别诊断在小型反刍动物CAPV暴发期间的疾病。(C)2010爱思唯尔B.V.保留所有权利。
Sheep poxvirus (SPPV), Goat poxvirus (GTPV) and Lumpy skin disease virus (LSDV) are Capripoxviruses (CaPVs) responsible for causing severe poxvirus disease in sheep, goats and cattle, respectively. Serological differentiation of CaPVs is not possible and strain identification has relied on the implicitly accepted hypothesis that the viruses show well defined host specificity. However, it is now known that cross infections can occur and authentication of identity based on the host animal species from which the strain was first isolated, is not valid and should be replaced with molecular techniques to allow unequivocal strain differentiation.To identify a diagnostic target for strain genotyping, the CaPV homologue of the Vaccinia virus E4L gene which encodes the 30 kDa DNA-dependant RNA polymerase subunit, RPO30 was analyzed. Forty-six isolates from different hosts and geographical origins were included. Most CaPVs fit into one of the three different groups according to their host origins: the SPPV, the GTPV and the LSDV group. A unique 21-nucleotide deletion was found in all SPPV isolates which was exploited to develop a RPO30-based classical PCR test to differentiate SPPV from GTPV that will allow rapid differential diagnosis of disease during CaPV outbreaks in small ruminants. (C) 2010 Elsevier B.V. All rights reserved.