Gene delivery into isolated Arabidopsis thaliana protoplasts and intact leaves using cationic, alpha-helical polypeptide
Gene delivery into isolated Arabidopsis thaliana protoplasts and intact leaves using cationic, alpha-helical polypeptide
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使用阳离子α-螺旋多肽将基因递送到分离的拟南芥原生质体和完整叶子中
DOI：
10.1007/s11705-017-1612-8
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发表时间：
2017
影响因子：
4.5
通讯作者：
Jianjun Cheng
中科院分区：
文献类型：
--
作者：
Nan Zheng;Ziyuan Song;Yang Liu;Lichen Yin;Jianjun Cheng
The application of gene delivery materials has been mainly focused on mammalian cells while rarely extended to plant engineering. Cationic polymers and lipids have been widely utilized to efficiently deliver DNA and siRNA into mammalian cells. However, their application in plant cells is limited due to the different membrane structures and the presence of plant cell walls. In this study, we developed the cationic,α-helical polypeptide that can effectively deliver DNA into both isolatedArabidopsis thalianaprotoplasts and intact leaves. The PPABLG was able to condense DNA to form nanocomplexes, and they exhibited significantly improved transfection efficiencies compared with commercial transfection reagent Lipofectamine 2000 and classical cell penetrating peptides such as poly(L-lysine), HIV-TAT, arginine9, and poly(L-arginine). This study therefore widens the utilities of helical polypeptide as a unique category of gene delivery materials, and may find their promising applications toward plant gene delivery.
