Effect of an anti-murine transferrin receptor-ricin A conjugate on bone marrow stem and progenitor cells treated in vitro.
Effect of an anti-murine transferrin receptor-ricin A conjugate on bone marrow stem and progenitor cells treated in vitro.
复制标题
抗鼠转铁蛋白受体-蓖麻毒素 A 缀合物对体外处理的骨髓干细胞和祖细胞的影响。
DOI：
10.1016/0014-4827(84)90583-4
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发表时间：
1984
影响因子：
3.7
通讯作者：
Trowbridge,IS
中科院分区：
文献类型：
--
作者：
Lesley,J;Domingo,DL;Schulte,R;Trowbridge,IS
研究了蓖麻毒素A亚基与小鼠转铁蛋白受体特异性单克隆抗体的结合。在小鼠t -淋巴瘤细胞株BW5147上测定了该偶联物的剂量范围、特异性和抑制蛋白合成的动力学。当毒素在整个培养过程中存在时，体外髓系（CFUc）和红细胞（CFUe和BFUe）骨髓菌落被偶联物抑制的剂量与小鼠细胞系中抑制蛋白质合成的剂量相当（ic50为5 × 10−11M）。将骨髓短暂暴露于抗转铁蛋白受体抗体-蓖麻毒素A偶联物中（30分钟至6小时），在体外检测骨髓（CFUc）和红细胞（CFUe和BFUe）祖细胞以及体内脾脏集落形成（cfu）。只有CFUe被这种脉冲暴露耗尽，这与CFUe群体中增殖细胞和转铁蛋白受体表达的频率相对于其他祖细胞更高一致。
A monoclonal antibody with specificity for murine transferrin receptor was conjugated with the toxic A subunit of ricin. The dose range, specificity, and kinetics of inhibition of protein synthesis of the conjugate were determined on the murine T-lymphoma cell line, BW5147. When toxin was present throughout the period of culture, in vitro myeloid (CFUc) and erythroid (CFUe and BFUe) bone marrow colonies were inhibited by doses of conjugate comparable to those that inhibit protein synthesis in murine cell lines (IC50of 5 × 10−11M). Bone marrow exposed briefly (30 min to 6 h) to anti-transferrin receptor antibody-ricin A conjugate was assayed for myeloid (CFUc) and erythroid (CFUe and BFUe) progenitors in vitro and for in vivo spleen colony formation (CFUs). Only CFUe were depleted by this pulse exposure, consistent with the higher frequency of proliferating cells and transferrin receptor expression in the CFUe population relative to other progenitors.