Identification of the Arabidopsis REDUCED DORMANCY 2 gene uncovers a role for the polymerase associated factor 1 complex in seed dormancy.
Identification of the Arabidopsis REDUCED DORMANCY 2 gene uncovers a role for the polymerase associated factor 1 complex in seed dormancy.
复制标题
拟南芥 REDUCED DORMANCY 2 基因的鉴定揭示了聚合酶相关因子 1 复合物在种子休眠中的作用
DOI：
10.1371/journal.pone.0022241
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Soppe WJ
中科院分区：
文献类型：
--
作者：
Liu Y;Geyer R;van Zanten M;Carles A;Li Y;Hörold A;van Nocker S;Soppe WJ
植物的生命以主要的相变为特征。这包括从种子到幼苗（发芽）和从营养生长到生殖生长（开花诱导）在农业上的重要转变。在包括拟南芥在内的许多植物物种中，刚收获的种子处于休眠状态，无法发芽。在休眠解除和有利环境条件出现后，萌发即可发生。虽然激素对种子休眠的控制已经得到了很好的研究，但诱导和释放休眠的分子机制尚不清楚。在这项研究中，我们报道了突变体减少休眠2-1 （rdo2-1）的克隆和特征。我们发现RDO2是最近发现的休眠基因TFIIS的等位基因，这是一个转录延伸因子。HUB1，以前被称为RDO4，在与rdo2-1相同的突变筛选中被鉴定为减少休眠，并且也被证明参与转录伸长。RDO2和HUB1的人类同源物与RNA聚合酶II相关因子1复合物（PAF1C）相互作用。因此，我们研究了其他拟南芥PAF1C相关因素的影响；VIP4, VIP5, ELF7， ELF8和ATXR7对种子休眠的影响。这些基因的突变导致休眠减少，类似于hub1-2和rdo2-1。与在种子成熟末期的作用一致，我们发现HUB1、RDO2和VIP5在这一发育阶段上调。由于PAF1C相关因子的突变体也被描述为提前开花，我们得出结论，这些成分参与了植物两个主要发育转变的调节。
The life of a plant is characterized by major phase transitions. This includes the agriculturally important transitions from seed to seedling (germination) and from vegetative to generative growth (flowering induction). In many plant species, including Arabidopsis thaliana, freshly harvested seeds are dormant and incapable of germinating. Germination can occur after the release of dormancy and the occurrence of favourable environmental conditions. Although the hormonal control of seed dormancy is well studied, the molecular mechanisms underlying the induction and release of dormancy are not yet understood. In this study, we report the cloning and characterization of the mutant reduced dormancy 2-1 (rdo2-1). We found that RDO2 is allelic to the recently identified dormancy gene TFIIS, which is a transcription elongation factor. HUB1, which was previously called RDO4, was identified in the same mutagenesis screen for reduced dormancy as rdo2-1 and was also shown to be involved in transcription elongation. The human homologues of RDO2 and HUB1 interact with the RNA Polymerase II Associated Factor 1 Complex (PAF1C). Therefore, we investigated the effect of other Arabidopsis PAF1C related factors; VIP4, VIP5, ELF7, ELF8 and ATXR7 on seed dormancy. Mutations in these genes resulted in reduced dormancy, similar to hub1-2 and rdo2-1. Consistent with a role at the end of seed maturation, we found that HUB1, RDO2 and VIP5 are upregulated during this developmental phase. Since mutants in PAF1C related factors are also described to be early flowering, we conclude that these components are involved in the regulation of both major developmental transitions in the plant.