Phylogenetic relationship of two popular edible Pleurotus in China, Bailinggu (P-eryngii var. tuoliensis) and Xingbaogu (P-eryngii), determined by ITS, RPB2 and EF1α sequences
Phylogenetic relationship of two popular edible Pleurotus in China, Bailinggu (P-eryngii var. tuoliensis) and Xingbaogu (P-eryngii), determined by ITS, RPB2 and EF1α sequences
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DOI：
10.1007/s11033-016-3982-2
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发表时间：
2016-06-01
影响因子：
2.8
通讯作者：
Gan, Bing-Cheng
中科院分区：
文献类型：
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作者：
He, Xiao-Lan;Wu, Bo;Gan, Bing-Cheng
本研究的目的是评估内部转录间隔区(ITS)、部分翻译延伸因子(EF1α)和RNA聚合酶II(RPB2)基因在白灵菇、杏鲍菇和白纹伊蚊中的应用；重建这三个物种之间的系统发育关系；并根据核糖体和蛋白质编码基因确定白灵菇的分类地位。利用p-距离模型计算了白灵菇、杏鲍菇和侧耳近缘菌株之间的遗传距离，并基于ITS、RPB2和EF1α，用最大简约法和贝叶斯方法估计了这些菌株的分子系统发育。ITS、RPB2和EF1α序列的差异表明，白灵菇、杏鲍鱼和白纹伊蚊在物种水平上是不同的。系统发育分析表明，杏鲍鱼与白灵菇的亲缘关系较近，而与白灵菇的亲缘关系较近。核糖体和蛋白质编码基因的序列分析证实，杏仁假单胞菌变种。托力斯与白灵谷完全相同。红豆杉P.eryngii var.在对新疆阿魏菌属的侧耳菌株进行深入调查后，应将托里氏菌种提升到种水平，或引入白灵菇新名称。本研究有助于解决白灵谷、杏仁假单胞菌和白纹假单胞菌的不确定性，改善这些菌株的资源管理。ITS、EF1α和RPB2序列可用于区分白令谷、杏鲍鱼和白纹伊蚊为三个不同的物种，以及杏鲍鱼变种。托里古斯应该是目前白灵谷的学名。
The aims of this study are to assess the utility of the internal transcribed spacer (ITS) region, and partial translation elongation factor (EF1 alpha) and RNA polymerase II (RPB2) genes, for differentiation of Bailinggu, P. eryngii, and P. nebrodensis; to reconstruct phylogenetic relationships between the three species; and to confirm the taxonomic status of Bailinggu based on ribosomal and protein-coding genes. Pairwise genetic distances between Bailinggu, P. eryngii, and related Pleurotus strains were calculated by using the p-distance model, and molecular phylogeny of these isolates was estimated based on ITS, RPB2, and EF1 alpha using maximum parsimony and Bayesian methods. Differences in ITS, RPB2, and EF1 alpha sequences show that Bailinggu, P. eryngii, and P. nebrodensis are distinct at the species level. Phylogenetic analyses reveal that P. eryngii is closer to P. nebrodensis than to Bailinggu. Sequence analyses of ribosomal and protein-coding genes confirm that P. eryngii var. tuoliensis is identical to Bailinggu. P. eryngii var. tuoliensis should be raised to species level or a new name should be introduced for Bailinggu after a thorough investigation into Pleurotus isolates from Ferula in Xinjiang Province. This study helps to resolve uncertainty regarding Bailinggu, P. eryngii and P. nebrodensis, improving the resource management of these strains. ITS, EF1 alpha, and RPB2 sequences can be used to distinguish Bailinggu, P. eryngii and P. nebrodensis as three different species, and P. eryngii var. tuoliensis should be the scientific name for Bailinggu at present.