Disassociation of glutamate from γ-aminobutyric acid by zinc acetate-assisted differential precipitation/dissolution: Application to the quantification of γ-aminobutyric acid
Disassociation of glutamate from γ-aminobutyric acid by zinc acetate-assisted differential precipitation/dissolution: Application to the quantification of γ-aminobutyric acid
复制标题
醋酸锌辅助微分沉淀/溶解从γ-氨基丁酸中解离谷氨酸：在γ-氨基丁酸定量中的应用
DOI：
10.1016/j.chroma.2019.01.002
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发表时间：
2019-04-12
影响因子：
4.1
通讯作者：
Wang, Shuixing
中科院分区：
文献类型：
--
作者：
Chen, Yuanhong;Chang, Kunpeng;Wang, Shuixing
γ-氨基丁酸(GABA)是生物体内一种重要的生理活性分子。从谷氨酸脱羧基产物中分离谷氨酸一直是研究的热点。这两种化合物之间的相互作用严重阻碍了它们的解离。在这里，我们提出了一种新的策略，称为醋酸锌辅助差异沉淀/溶解(ZA-DPD)，通过逐步回收纯GABA溶液和丢弃纯谷氨酸颗粒来去除谷氨酸，主要归因于使用两种核心试剂(醋酸锌辅助谷氨酸沉淀剂和谷氨酸排斥试剂)。在每一次沉淀中，醋酸锌辅助的谷氨酸沉淀剂保证了大多数GABA仍然是可溶的，尽管其余的GABA与谷氨酸共沉淀；在耦合溶解中，共沉淀的GABA在有或没有谷氨酸共溶解的情况下完全溶解(在最终溶解中使用的是谷氨酸排斥试剂)。这个过程重复两次，直到谷氨酸被彻底去除。建立了ZA-DPD与比色法联用测定GABA的准确定量方法。这项研究可能有助于与GABA或谷氨酸相关的区域的研究。(C)2019爱思唯尔B.V.保留所有权利。
gamma-aminobutyric acid (GABA) is a key physiologically active molecule in organisms. Separation of glutamate from its decarboxylated product GABA has been vigorously pursued. The interaction between these two compounds severely hindered their disassociation. Herein, we present a new strategy, termed zinc acetate-assisted differential precipitation/dissolution (ZA-DPD), for the removal of glutamate by step by step recovering pure GABA solution and discarding pure glutamate pellet, essentially attributed to the use of two core reagents (zinc acetate-assisted glutamate-precipitating reagent, and glutamate-rejecting reagent). In each precipitation, the zinc acetate-assisted glutamate-precipitating reagent guaranteed most GABA still soluble although the rest co-precipitated with glutamate; in the coupled dissolution, the co-precipitated GABA was fully dissolved with or without (in the case of glutamate-rejecting reagent used in the final dissolution) co-dissolution of glutamate. The process was repeated twice until glutamate was thoroughly removed. An accurate quantitative method coupling ZA-DPD with colorimetry was thereafter established for the determination of GABA. This study may facilitate the areas associated with GABA or glutamate. (C) 2019 Elsevier B.V. All rights reserved.