Detecting Necroptosis in Virus-Infected Cells.
Detecting Necroptosis in Virus-Infected Cells.
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DOI：
10.1007/978-1-0716-1012-1_11
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Jacobs BL
中科院分区：
文献类型：
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作者：
Cotsmire SM;Szczerba M;Jacobs BL
坏死性凋亡被认为是癌症、阿尔茨海默病和其他神经退行性疾病以及病毒感染细胞中的关键细胞死亡途径。当混合谱系激酶结构域样蛋白 (MLKL) 刺穿细胞质膜，导致水快速流入，导致细胞完整性丧失时，就会发生坏死性凋亡。随着其在人类疾病中的作用变得越来越明显，识别坏死性凋亡的方法需要进一步开发和优化。在这里，我们描述了通过使用通路抑制剂量化细胞死亡来识别坏死性凋亡，并使用蛋白质印迹来识别 MLKL 激活的终点以及导致其的蛋白质-蛋白质相互作用。
Necroptosis has been implicated as a critical cell death pathway in cancers, Alzheimer’s and other neurodegenerative diseases, and virus-infected cells. Necroptosis occurs when mixed-lineage kinase domain-like protein (MLKL) punctures the cytoplasmic membrane allowing a rapid influx of water leading to a loss of cellular integrity. As its role in human disease becomes apparent, methods identifying necroptosis will need to be further developed and optimized. Here we describe identification of necroptosis through quantifying cell death with pathway inhibitors and using western blots to identify end points of MLKL activation and protein-protein interactions leading to it.