Distribution patterns of HP1, a heterochromatin-associated nonhistone chromosomal protein of Drosophila.
Distribution patterns of HP1, a heterochromatin-associated nonhistone chromosomal protein of Drosophila.
复制标题
DOI：
--
复制
发表时间：
1989-10
影响因子：
6.6
通讯作者：
T. James;J. C. Eissenberg;C. Craig;V. Dietrich;A. Hobson;Sarah C. R. Elgin
中科院分区：
文献类型：
--
作者：
T. James;J. C. Eissenberg;C. Craig;V. Dietrich;A. Hobson;Sarah C. R. Elgin
我们以前曾报道的非组蛋白染色体蛋白（nhcp-19，现在称为HP 1）优先与异染色质的果蝇的鉴定。用单克隆抗体C1 A9免疫荧光染色，对HP 1在多线染色体上的分布模式进行了详细的研究。结果表明，这种蛋白质被发现在中心β异染色质，在细胞学区域31，41和80，并在整个多线染色体4。端粒的染色是经常观察到的，染色体臂2 R和3R以及X染色体的染色最明显。第四染色体插入易位T（3;4）f/In（3L）P的分析证实了与4号染色体材料的自主相互作用。类似地，在重排ltx 13中移动到染色体臂3R上的位置97 D2的染色体臂2L上的光远端的β-异染色质使用C1 A9抗体显著染色。完整唾液腺的染色表明，β-异染色质的重排片段与多线染色中心无关，但提供了一个独立的结构参考点。在早期合胞体胚胎的细胞核中没有观察到HP 1，但在合胞体胚盘阶段（约100年），HP 1在细胞核中变得集中。核分裂周期10）。这表明，异染色质的形成发生在大约相同的阶段，在细胞核第一次成为转录能力。因此，C1 A9抗体可以作为一个有用的标记果蝇细胞核的结构和功能的研究。
We have previously reported the identification of a nonhistone chromosomal protein (nhcp-19; now called HP1) preferentially associated with the heterochromatin of Drosophila melanogaster. A detailed study of the HP1 distribution pattern on polytene chromosomes by immunofluorescent staining, using monoclonal antibody C1A9, has been carried out. The results indicate that this protein is found within the centric beta-heterochromatin, in cytological regions 31, 41 and 80, and throughout polytene chromosome 4. Staining of telomeres is frequently observed, those of chromosome arms 2R and 3R and the X chromosome being the most conspicuous. Analysis of a fourth chromosome insertional translocation T(3;4)f/In(3L)P confirms an autonomous interaction with chromosome 4 material. Similarly, the beta-heterochromatin distal to light on chromosome arm 2L, moved to position 97D2 on chromosome arm 3R in the rearrangement ltx13, is prominently stained using the C1A9 antibody. Staining of intact salivary glands indicates that this rearranged segment of beta-heterochromatin is not associated with the polytene chromocenter, but provides an independent structural reference point. HP1 is not observed in the nuclei of the early syncytial embryo, but becomes concentrated in the nuclei at the syncytial blastoderm stage (ca. nuclear division cycle 10). This suggests that heterochromatin formation occurs at approximately the same stage at which nuclei first become transcriptionally competent. Thus, the C1A9 antibody may serve as a useful marker for both structural and functional studies of the Drosophila nucleus.