Intracellular replication of Salmonella within epithelial cells is associated with filamentous structures containing lysosomal membrane glycoproteins.
Intracellular replication of Salmonella within epithelial cells is associated with filamentous structures containing lysosomal membrane glycoproteins.
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沙门氏菌在上皮细胞内的细胞内复制与含有溶酶体膜糖蛋白的丝状结构有关。
DOI：
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
B. Finlay
中科院分区：
文献类型：
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作者：
F. Garcia;Michael B. Zwick;Leung Ky;B. Finlay
我们已经研究了S的目标。在侵入培养的HeLa上皮细胞后，含鼠伤寒沙门氏菌的空泡转化为溶酶体。我们的研究结果表明，胞内细菌与含有溶酶体膜糖蛋白（LGP）的空泡共定位。人LGP hlamp-1和hlamp-2均存在于S.从感染后约2 h开始出现含鼠伤寒嘧啶的空泡。在稍后的时间（4-6小时），长而稳定的丝状结构与溶酶体标志物出现连接到感染细胞中的含细菌的空泡。这些结构的形成需要活的细胞内细菌，这在未感染的细胞或感染另一种侵袭性细菌小肠结肠炎耶尔森氏菌的HeLa上皮细胞中是检测不到的。动力学分析表明，这些LGP丰富的细丝的外观和细胞内细菌复制的启动之间的严格相关性。此外，这些结构在感染某些S.鼠伤寒杆菌细胞内复制缺陷突变体。额外的数据证实，一个完整的微管网络和intravacuolar酸性pH值是必需的，以诱导含LGP的丝状结构的形成，这些结构的形态和功能不同于先前描述的管状溶酶体。
We have examined the targeting of S. typhimurium-containing vacuoles to lysosomes after invasion of cultured HeLa epithelial cells. Our results show that intracellular bacteria colocalize with vacuoles containing lysosomal membrane glycoproteins (LGPs). Both human LGPs, hlamp-1 and hlamp-2, are present in S. typhimurium-containing vacuoles from approximately 2 h postinfection. At later times (4-6 h), long and stable filamentous structures with lysosomal markers appear connected to bacteria-containing vacuoles in infected cells. Viable intracellular bacteria are required for the formation of these structures, which are not detected in uninfected cells or in HeLa epithelial cells infected with another invasive bacteria, Yersinia enterocolitica. Kinetics analysis showed a strict correlation between the appearance of these LGP-rich filaments and the initiation of intracellular bacterial replication. Moreover, these structures are absent in epithelial cells infected with certain S. typhimurium intracellular replication-defective mutants. Additional data confirmed that an intact microtubule network and intravacuolar acidic pH are required to induce the formation of LGP-containing filamentous structures and that these structures are morphologically and functionally different from previously described tubular lysosomes.