Patterning the surface cytophobicity of an albumin-physisorbed substrate by electrochemical means
Patterning the surface cytophobicity of an albumin-physisorbed substrate by electrochemical means
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DOI：
10.1021/la050660n
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发表时间：
2005-07-19
期刊：
影响因子：
3.9
通讯作者：
Nishizawa, M
中科院分区：
文献类型：
--
作者：
Kaji, H;Tsukidate, K;Nishizawa, M
在生理条件下对表面特性的时空控制是基础细胞生物学、组织工程和基于细胞的生物电子学中的一项重要技术。为此，我们开发了一种温和的湿细胞微图案技术。该技术的原理是基于这样一个事实，即白蛋白涂层基底的细胞排斥特性在暴露于活性氧化剂（电化学生成的次溴酸）时迅速转变为细胞粘附。在此，我们报告了次溴酸对在疏水底物上物理吸收的血清白蛋白的影响。研究发现，白蛋白分子通过使用氧化剂从底物上分离，导致底层疏水表面暴露在液相中。细胞外基质蛋白如纤维连接蛋白在疏水表面的吸附可诱导细胞粘附和生长。
Spatiotemporal control of surface properties under physiological conditions such as those found in culture media is an important technique in fundamental cell biology, tissue engineering, and cell-based bioelectronics. To this end, we have developed a mild, wet cellular micropatterning technique. The principle of the technique is based on the fact that the cell-repellant property of the albumin-coated substrate rapidly switches to cell-adhesive upon exposure to the reactive oxidizing agent, electrochemically generated hypobromous acid. Herein, we report the effect of the hypobromous acid on serum albumin physisorbed on a hydrophobic substrate. It was found that albumin molecules detach from the substrate by application of the oxidizing agent, resulting in exposure of the underlying hydrophobic surface to the liquid phase. The adsorption of extracellular matrix proteins such as fibronectin onto the hydrophobic surface induces cell adhesion and growth.