LONG-TERM EFFECTS OF A SINGLE DOSE OF ULTRAVIOLET-B ON ALBINO RABBIT CORNEA .1. INVIVO ANALYSES
LONG-TERM EFFECTS OF A SINGLE DOSE OF ULTRAVIOLET-B ON ALBINO RABBIT CORNEA .1. INVIVO ANALYSES
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DOI：
10.1111/j.1751-1097.1989.tb04095.x
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发表时间：
1989-02-01
影响因子：
3.3
通讯作者：
CULLEN, AP
中科院分区：
文献类型：
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作者：
DOUGHTY, MJ;CULLEN, AP
雌性白化兔（1.9 kg）的双眼在13.30至15.00小时期间暴露于单剂量UV-B（300±9nm；0.125J/cm2总剂量）。平均辐照度为209.+-。 4 .mu.W/cm2 交付超过 612 .+-。 13秒。此后的不同时间段（照射后3天、6、7、14、28、42、56、112、224和336天每12小时）对动物进行全裂隙灯检查以评估角膜和眼前节的状态以及中央角膜厚度的光学或超声测厚仪。研究结果与同期年龄匹配的兔子的研究进行了比较。在 UV-B 照射下，角膜出现适度水肿（中央角膜厚度增加 20%），并在 48 小时达到峰值。中央角膜厚度在 6 天内恢复到接近正常，随后出现继发性非常轻微的肿胀 (< 5%)，并在 14 天后消退。同期水肿伴有角膜炎。此后，对照和 UV-B 照射的角膜厚度都随着年龄的增长而逐渐增加。生物显微镜还显示角膜上皮中出现颗粒状混浊，该混浊在 72-96 小时达到峰值，并在 28 天内消退。此外，UV-B 照射的角膜中存在非常小的角膜上皮微粒混浊，该混浊在 72 小时时达到最大值，但在整个评估期间在某种程度上持续存在。生物显微镜还显示，角膜基质的均匀性逐渐受到破坏，出现了大的“面包屑”状混浊，这种混浊从 72 小时开始，在评估期结束时仍然存在。这些结果表明，急性 UV-B 暴露后对角膜的长期评估非常重要，并表明急性 UV-R 暴露可能会产生与长期暴露于富含 UV-R 的环境后报告的角膜变化类似的变化。
Both eyes of female albino rabbits (1.9 kg) were exposed to a single dose of UV-B (300 .+-. 9 nm; 0.125 J/cm2 total dose) between 13.30 and 15.00 h. The average irradiance was 209 .+-. 4 .mu.W/cm2 delivered over 612 .+-. 13 s. At various time periods thereafter (every 12 h for 3 days, 6, 7, 14, 28, 42, 56, 112, 224 and 336 days post-irradiation), the animals were subjected to a full slit lamp examination to evaluate the status of the cornea and the anterior segment along with optical or ultrasonic pachometry of central corneal thickness. The results were compared with studies on age-matched rabbits over the same time period. In response to the UV-B irradiation, the corneas showed a modest edema (20% increase in central corneal thickness) that peaked at 48 h. Nearly normal central corneal thickness returned in 6 days and followed by a secondary very slight swelling (< 5%) that resolved by 14 days. The edema was accompanied by keratitis over the same period. Thereafter, both control and UV-B irradiated corneas progressively increased in thickness with age. Biomicroscopy also revealed the appearance of granular opacities in the corneal epithelium that peaked at 72-96 h and resolved over 28 days. In addition, very small microdot opacities of the corneal epithelium were present in the UV-B irradiated corneas that reached maximum at 72 h but persisted to some degree throughout the evaluation period. Biomicroscopy also revealed a progressive disruption of the homogeneous nature of the corneal stroma by the appearance of large ''bread crumb''-like opacities that started at 72 h and was still present at the end of the evaluation period. These results suggest that long-term evaluation of the cornea is important after acute UV-B exposure and indicate that acute exposure to UV-R can product corneal changes resembling those reported following chronic exposure to UV-R-rich environments.