ZomB is essential for chemotaxis of Vibrio alginolyticus by the rotational direction control of the polar flagellar motor
ZomB is essential for chemotaxis of Vibrio alginolyticus by the rotational direction control of the polar flagellar motor
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DOI：
10.1111/gtc.12895
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发表时间：
2021-09-27
期刊：
影响因子：
2.1
通讯作者：
Homma, Michio
中科院分区：
文献类型：
--
作者：
Takekawa, Norihiro;Nishikino, Tatsuro;Homma, Michio
细菌通过控制鞭毛的旋转向喜欢的地方移动或远离不喜欢的地方来显示趋化性。这种旋转的变化是由趋化信号蛋白chey -磷酸（CheY-P）与鞭毛马达中的c环结合引起的。一些特殊的细菌，包括弧菌和希瓦氏菌，有一种叫做ZomB的跨膜蛋白。ZomB对于控制腐坏希瓦氏菌和副溶血性弧菌的鞭毛旋转方向至关重要。在这项研究中，我们证实了zomB的缺失只导致藻酸溶解弧菌的马达逆时针（CCW）旋转，正如之前在其他细菌中报道的那样。我们发现，由fliG和fliM突变引起的顺时针锁定表型不需要ZomB，而由CheY-P过量产生引起的CW旋转则需要ZomB。纯化后的ZomB蛋白形成多聚体，表明ZomB可能具有同型寡聚物的功能。这些观察结果表明，ZomB与鞭毛运动旋转、趋化性或两者兼而有之的蛋白相互作用。我们提供的证据表明，ZomB在趋化性中是一个新的参与者，并且除了CheY外，还需要在溶藻弧菌中进行旋转控制。
Bacteria exhibit chemotaxis by controlling flagellar rotation to move toward preferred places or away from nonpreferred places. The change in rotation is triggered by the binding of the chemotaxis signaling protein CheY-phosphate (CheY-P) to the C-ring in the flagellar motor. Some specific bacteria, including Vibrio spp. and Shewanella spp., have a single transmembrane protein called ZomB. ZomB is essential for controlling the flagellar rotational direction in Shewanella putrefaciens and Vibrio parahaemolyticus. In this study, we confirmed that the zomB deletion results only in the counterclockwise (CCW) rotation of the motor in Vibrio alginolyticus as previously reported in other bacteria. We found that ZomB is not required for a clockwise-locked phenotype caused by mutations in fliG and fliM, and that ZomB is essential for CW rotation induced by overproduction of CheY-P. Purified ZomB proteins form multimers, suggesting that ZomB may function as a homo-oligomer. These observations imply that ZomB interacts with protein(s) involved in either flagellar motor rotation, chemotaxis, or both. We provide the evidence that ZomB is a new player in chemotaxis and is required for the rotational control in addition to CheY in Vibrio alginolyticus.