Triggering the biocontrol of Botrytis cinerea by Trichoderma harzianum through inhibition of pathogenicity and virulence related proteins
Triggering the biocontrol of Botrytis cinerea by Trichoderma harzianum through inhibition of pathogenicity and virulence related proteins
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DOI：
10.15302/j-fase-2018214
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发表时间：
2018-05
影响因子：
3.7
通讯作者：
K. Saravanakumar;Zhixiang Lu;H. Xia;Meng Wang;Jianan Sun;Shaoqing Wang;Qiang-qiang Wang;Li Yaqi
中科院分区：
文献类型：
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作者：
K. Saravanakumar;Zhixiang Lu;H. Xia;Meng Wang;Jianan Sun;Shaoqing Wang;Qiang-qiang Wang;Li Yaqi
本研究报道了一株哈茨木霉（Trichoderma harzianum）菌株CCTCC-SBW 0162，其具有增强对灰霉病病原菌灰葡萄孢（Botrytis cinerea）的生防活性的潜力，并且在番茄（Solanum esculentum）植物生长促进中具有关键作用。采用体外拮抗试验对254株木霉菌进行了筛选。其中，10个被选择用于温室实验，基于它们对B的更大抑制。灰叶离体拮抗试验和温室盆栽试验表明，T。哈茨菌CCTCC-SBW 0162的抑菌率和防病率最高，分别为90.6%和80.7%。研究CCTCC-SBW 0162对B的抑菌作用机制。利用分子对接技术研究了CCTCC-SBW 0162代谢产物与B致病性和毒力相关蛋白的相互作用。灰叶分子对接结果表明，CCTCCSBW 0162中的harzianopyridone、harzianopyridone和蒽醌C的组合可协同提高抗B的活性。灰霉病菌通过抑制/修饰致病性和毒力相关蛋白。然而，这一计算机模拟工作强调需要在实验室中进行进一步的研究，以确认T。针对B的蛋白质的哈茂衍生代谢物。灰岩及其相互作用
This study reports a strain of Trichoderma harzianum CCTCC-SBW0162 with potential to enhance biocontrol activity against gray mold pathogen, Botrytis cinerea, and with a pivotal role in tomato (Solanum esculentum) plant growth enhancement. A total of 254 Trichoderma isolates were screened by in vitro antagonistic assay. Of these, 10 were selected for greenhouse experiments based on their greater inhibition of B. cinerea. The in vitro antagonistic assay and greenhouse experiments indicated that T. harzianum CCTCC-SBW0162 gave the highest inhibition rate (90.6%) and disease reduction (80.7%). Also, to study the possible mechanism associated with antifungal activity of CCTCC-SBW0162 against B. cinerea, molecular docking was used to assess the interactions between CCTCC-SBW0162-derived metabolites, and pathogencity and virulence related proteins of B. cinerea. The molecular docking results indicated that the combination of harzianopyridone, harzianolide and anthraquinone C derived from CCTCCSBW0162 could synergistically improve antifungal activity against B. cinerea through the inhibition/modification of pathogenicity and virulence related proteins. However, this computerized modeling work emphasized the need for further study in the laboratory to confirm the effect T. harzianum-derived metabolites against the proteins of B. cinerea and their interactions.