Effects of a proteasome inhibitor on the riboflavin production in <i>Ashbya gossypii</i>
Effects of a proteasome inhibitor on the riboflavin production in <i>Ashbya gossypii</i>
复制标题
蛋白酶体抑制剂对棉麻核黄素产生的影响
DOI：
10.1111/jam.15296
复制
发表时间：
2021
影响因子：
4
通讯作者：
Park Enoch Y.
中科院分区：
文献类型：
--
作者：
Kato Tatsuya;Yokomori Ami;Suzuki Riho;Azegami Junya;El Enshasy Hesham A.;Park Enoch Y.
目的研究蛋白酶体抑制剂MG-132对棉阿舒囊菌核黄素合成的影响，以阐明核黄素合成与黄素蛋白稳态的关系。方法和结果在25 μM浓度下，添加MG-132 24 h后，棉阿舒囊菌核黄素合成减少了79%。抑制剂的加入还引起活性氧和泛素化蛋白的积累。这些结果表明，MG-132在没有任何基因工程的情况下在棉曲霉中起作用，并减少核黄素的产生。在存在25 μM MG-132的情况下，与DMSO相比，比NADH脱氢酶活性增加1.4倍，但与DMSO相比，比琥珀酸脱氢酶（SDH）活性降低至52%。此外，AgSdh 1 p（ACR 052 Wp）的量也减少。特定的核黄素生产减少到22%时，20 mM的丙二酸，SDH抑制剂，加入到培养基中。异源AgSDH 1基因破坏突变体（AgSDH 1-/+）的核黄素产量比野生型降低了63%。结论MG-132可抑制棉曲霉的核黄素产量和SDH活性。SDH是参与核黄素合成的黄素蛋白之一。本研究的意义和影响本研究表明MG-132对棉曲霉的核黄素合成和SDH活性有负面影响，并阐明了核黄素合成与黄素蛋白的关系。
AimsEffects of a proteasome inhibitor, MG‐132, on the riboflavin production inAshbya gossypiiwere investigated to elucidate the relationship of the riboflavin production with flavoprotein homeostasis.Methods and ResultsThe addition of MG‐132 to the liquid medium reduced the specific riboflavin production by 79% inA.gossypiiat 25 μM after 24 h. The addition of the inhibitor also caused the accumulation of reactive oxygen species and ubiquitinated proteins. These results indicated that MG‐132 works inA.gossypiiwithout any genetic engineering and reduces riboflavin production. In the presence of 25 μM MG‐132, specific NADH dehydrogenase activity was increased by 1.4‐fold compared to DMSO, but specific succinate dehydrogenase (SDH) activity was decreased to 52% compared to DMSO. Additionally, the amount of AgSdh1p (ACR052Wp) was also reduced. Specific riboflavin production was reduced to 22% when 20 mM malonate, a SDH inhibitor, was added to the culture medium. The riboflavin production in heterozygousAgSDH1gene‐disrupted mutant (AgSDH1−/+) was reduced to 63% compared to that in wild type.ConclusionsMG‐132 suppresses the riboflavin production and SDH activity inA.gossypii. SDH is one of the flavoproteins involved in the riboflavin production inA.gossypii.Significance and Impact of the StudyThis study shows that MG‐132 has a negative influence on the riboflavin production and SDH activity inA.gossypiiand leads to the elucidation of the connection of the riboflavin production with flavoproteins.