Crystallization and preliminary X-ray analysis of Pac17 from the pacidamycin-biosynthetic cluster of Streptomyces coeruleorubidus.
Crystallization and preliminary X-ray analysis of Pac17 from the pacidamycin-biosynthetic cluster of Streptomyces coeruleorubidus.
复制标题
来自蓝链霉菌帕西霉素生物合成簇的 Pac17 的结晶和初步 X 射线分析。
DOI：
10.1107/s1744309112029144
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Tromans DR
中科院分区：
文献类型：
--
作者：
Tromans DR
Pac17 是来自土壤细菌蓝链霉菌 (Streptomyces coeruleorubidus) pacidamycin 基因簇的未表征蛋白质。它与抗生素核心二氨基丁酸残基的生物合成有关，尽管目前其确切作用尚不确定。鉴于帕西霉素可抑制铜绿假单胞菌的转位酶 I（临床上尚未开发的抗生素靶点），因此它们为寻找针对这种重要病原体的抗菌药物带来了新的希望。 Pac17 晶体通过蒸气扩散生长，并在同步加速器上从单晶收集分辨率为 1.9 Å 的 X 射线数据。该晶体属于C2空间群，晶胞参数a = 214.12，b = 70.88，c = 142.22 Å，β = 92.96°。对这些数据的初步分析表明，不对称单元由一个 Pac17 同四聚体组成，估计溶剂含量为 49.0%。
Pac17 is an uncharacterized protein from the pacidamycin gene cluster of the soil bacterium Streptomyces coeruleorubidus. It is implicated in the biosynthesis of the core diaminobutyric acid residue of the antibiotic, although its precise role is uncertain at present. Given that pacidamycins inhibit translocase I of Pseudomonas aeruginosa, a clinically unexploited antibiotic target, they offer new hope in the search for antibacterial agents directed against this important pathogen. Crystals of Pac17 were grown by vapour diffusion and X-ray data were collected at a synchrotron to a resolution of 1.9 Å from a single crystal. The crystal belonged to space group C2, with unit-cell parameters a = 214.12, b = 70.88, c = 142.22 Å, β = 92.96°. Preliminary analysis of these data suggests that the asymmetric unit consists of one Pac17 homotetramer, with an estimated solvent content of 49.0%.
影响因子：
4.9
作者：
Mueller, C.;Nolden, S.;Schwartz, D.
通讯作者：
Schwartz, D.
影响因子：
4.9
作者：
ISONO, F;INUKAI, M
通讯作者：
INUKAI, M