Heterogeneity of intact granules purified from canine mastocytoma cell lines.
Heterogeneity of intact granules purified from canine mastocytoma cell lines.
复制标题
从犬肥大细胞瘤细胞系纯化的完整颗粒的异质性。
DOI：
10.1152/ajplung.1991.260.2.l153
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Lazarus,SC
中科院分区：
文献类型：
--
作者：
Thomas,PS;Schreck,RE;Ruoss,SJ;Lazarus,SC
我们研究了一些肥大细胞表现出颗粒相关中性蛋白酶的异步释放的观察。完整的肥大细胞颗粒进行分离，纯化，并研究其组胺，中性蛋白酶，和蛋白多糖含量。对两种犬肥大细胞瘤细胞系的研究表明，在中性蛋白酶和密度方面，其中一种细胞系（G）内颗粒的储存和包装存在差异，导致两种不同的颗粒亚群。通过使用相同的技术，在其他细胞系（BR）中未发现此类差异。这些观察结果表明，在一些肥大细胞的颗粒亚群可能有一个不同的释放介质的作用。
We investigated the observation that some mast cells exhibit asynchronous release of granule-associated neutral proteases. Intact mast cell granules were isolated, purified, and studied with respect to their histamine, neutral protease, and proteoglycan content. Studies of two canine mastocytoma cell lines demonstrated differences in storage and packaging of granules within one of the cell lines (G) with respect to the neutral proteases and density, resulting in two distinct subpopulations of granules. By use of identical techniques no such differences were found in the other cell line (BR). These observations suggest that granule subpopulations in some mast cells may have a role in the differential release of mediators.