Validation of transonic small animal flowmeter for measurement of cardiac output and regional blood flow in the rat
Validation of transonic small animal flowmeter for measurement of cardiac output and regional blood flow in the rat
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DOI：
10.1097/00005344-199604000-00005
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发表时间：
1996-04-01
影响因子：
3
通讯作者：
Whitworth, JA
中科院分区：
文献类型：
--
作者：
Wen, C;Li, M;Whitworth, JA
本研究的目的是验证两种探头(3SS型心输出量(CO)和1RB型器官血流)的跨音速流量计系统在SD大鼠中的有效性。首先，通过测量灌注泵的离体肾动脉和升主动脉的血流量，然后通过测量CO和活体肾、肠系膜、臀部血流量(RBF、MBF、HBF)。我们测量了在体血流量以及多巴胺和心得安对CO、前列腺素E(2)(PGE(2))、血管紧张素II(AII)对RBF和戊巴比妥钠对MBF和HBF引起的流量变化。流量计与泵流量呈线性相关(r=0.999，p<；0.001)，升主动脉和肾动脉流量测量结果基本一致。基础值分别为CO 15+/-0.7ml/100g/min、RBF 4+/-0.1ml/100g/min、MBF 7+/-0.3ml/100g/min、HBF 6+/-0.3ml/100g/min。该系统可靠地检测到CO和局部血流量的增加和/或减少。该跨音速流量计系统准确、重复性高，并与其他已建立的测量CO和大鼠局部血流的技术兼容。
The objective of the present study was to validate a transonic flowmeter system with two probes (model 3SS for cardiac output (CO) and 1RB for organ flows) in Sprague-Dawley (SD) rats first by measuring blood flow through pump-infused isolated renal artery and ascending aorta, and then through measurements of CO and renal, mesenteric, and hindquarter blood flow (RBF, MBF, HBF) in vivo. We measured in vivo baseline flow and changes in flow induced by dopamine and propranolol for CO, prostaglandin E(2) (PGE(2)), and angiotensin II (AII) for RBF and pentobarbital sodium for MBF and HBF. Correlations between meter and pump flow were linear (r = 0.999, p < 0.001) with close agreement both in ascending aorta and renal artery flow measurements. The baseline values were 15 +/- 0.7 ml/100 g/min for CO, 4 +/- 0.1 ml/100 g/min for RBF, 7 +/- 0.3 ml/100 g/min for MBF, and 6 +/- 0.3 ml/100 g/min for HBF, respectively. The system reliably detected increase and/or decrease in CO and regional blood flows. The transonic flowmeter system is accurate, highly reproducible, and compatible with other established techniques for measuring CO and regional blood flows in the rat.