Focal adhesion motility revealed in stationary fibroblasts
Focal adhesion motility revealed in stationary fibroblasts
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DOI：
10.1126/science.286.5442.1172
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发表时间：
1999-11-05
期刊：
影响因子：
56.9
通讯作者：
Gundersen, GG
中科院分区：
文献类型：
--
作者：
Smilenov, LB;Mikhailov, A;Gundersen, GG
粘着斑（Focal adhesions，FA）是一类聚集的整合素及其相关蛋白，介导细胞粘附和信号传导.用绿色荧光蛋白-PI整合素嵌合体标记活细胞中的FA。在静止的细胞中，FA是高度运动的，线性地向细胞中心移动几个空斑长度。FA运动是独立的细胞密度和相关的肌动蛋白纤维收缩的结果。在迁移细胞中，FA是静止的，仅在尾部移动。FA运动在静止的细胞表明，细胞运动可能是由离合器样机制，整合素的亲和力基板可能会改变响应迁移线索。
Focal adhesions (FAs) are clustered integrins and associated proteins that mediate cell adhesion and signaling. A green fluorescent protein-pi integrin chimera was used to label FAs in Living cells. in stationary cells, FAs were highly motile, moving linearly for several plaque lengths toward the cell center. FA motility was independent of cell density and resulted from contraction of associated actin fibers. In migrating cells, FAs were stationary and only moved in the tail. FA motility in stationary cells suggests that cell movement may be regulated by a clutch-like mechanism by which the affinity of integrins to substrate may be altered in response to migratory cues.