COBRA VENOM FACTOR - EVIDENCE FOR ITS BEING ALTERED COBRA C3 (3RD COMPONENT OF COMPLEMENT)
COBRA VENOM FACTOR - EVIDENCE FOR ITS BEING ALTERED COBRA C3 (3RD COMPONENT OF COMPLEMENT)
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DOI：
10.1126/science.56780
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发表时间：
1976-01-01
期刊：
影响因子：
56.9
通讯作者：
BALAVITCH, D
中科院分区：
文献类型：
--
作者：
ALPER, CA;BALAVITCH, D
有证据表明，眼镜蛇蛇毒因子[CVF]是眼镜蛇毒中的抗互补蛋白，是经过修饰的眼镜蛇C3。抗CVF交叉抗体[兔]与人C3发生反应。眼镜蛇血清中的一种蛋白与CVF的抗血清发生强烈的反应，前者与人C3一样，通过眼镜蛇血清与内毒素、联氨或简单储存在37℃下孵育而转化。C.CVF与眼镜蛇血清孵育可破坏人血清中CVF的C3裂解活性；人C3b[C3，b片段]灭活剂无效。CVF似乎是C3的一种形式(可能是C3b)；它在人血清中的强大作用可能来自于它对人类C3b灭活剂缺乏敏感性。
Evidence is presented that cobra venom factor [CVF], the anti-complementary protein in Naja naja venom, is modified cobra C3. Antiserum [rabbit] to the CVF cross reacts with human C3. A protein in cobra serum reacts strongly with antiserum to the CVF, and the former protein, like human C3, is converted by incubation of cobra serum with [Escherichia-coli] endotoxin, hydrazine or simple storage at 37.degree. C. Incubation of CVF with cobra serum destroys the C3 cleaving activity of the CVF in human serum; human C3b [C3, b fragment] inactivator is ineffective. The CVF appears to be a form of C3 (perhaps C3b); its potent action in human serum probably derives from its lack of sensitivity to human C3b inactivator.