Characterization of a copper-resistant symbiotic bacterium isolated from Medicago lupulina growing in mine tailings
Characterization of a copper-resistant symbiotic bacterium isolated from Medicago lupulina growing in mine tailings
复制标题
从尾矿中生长的紫花苜蓿中分离出的抗铜共生细菌的表征
DOI：
10.1016/j.biortech.2010.08.046
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发表时间：
2011-01-01
影响因子：
11.4
通讯作者：
Wei, Ge-Hong
中科院分区：
文献类型：
--
作者：
Fan, Lian-Mei;Ma, Zhan-Qiang;Wei, Ge-Hong
从尾矿中的苜蓿（Medicago lupulina）中分离到一株抗1.4 mM Cu 2+的根瘤菌，苜蓿中华根瘤菌（Sinorhizobium meliloti）CCNWSX 0020。在添加铜的培养基中，由于铜沉淀在细胞表面，该细菌表现出细胞变形和聚集。通过PCR从1.4 Mb的大质粒中扩增与来自大肠杆菌的铜抗性基因pcoR和pcoA相似的基因。接种S.苜蓿CCNWSX 0020提高了M.在添加0和100 mg Cu ~（2+）kg ~（-1）的培养基中，啤酒花的铜含量分别提高了45.8%和78.2%，而在添加100 μ M Cu ~（2+）的培养基中，啤酒花的铜含量提高了39.3%，表明啤酒花是一种有前景的生物修复共生体系。(C)2010爱思唯尔有限公司保留所有权利。
A root nodule bacterium, Sinorhizobium meliloti CCNWSX0020, resistant to 1.4 mM Cu2+ was isolated from Medicago lupulina growing in mine tailings. In medium supplied with copper, this bacterium showed cell deformation and aggregation due to precipitation of copper on the cell surface. Genes similar to the copper-resistant genes, pcoR and pcoA from Escherichia coli, were amplified by PCR from a 1.4-Mb mega-plasmid. Inoculation with S. meliloti CCNWSX0020 increased the biomass of M. lupulina grown in medium added 0 and 100 mg Cu2+ kg(-1) by 45.8% and 78.2%, respectively, and increased the copper concentration inside the plant tissues grown in medium supplied with 100 mu M Cu2+ by 39.3%, demonstrating that it is a prospective symbiotic system for bioremediation purposes. (C) 2010 Elsevier Ltd. All rights reserved.