Fine-Tuning the Characteristic of the Applied Potential To Improve AC-iEK Separations of Microparticles
Fine-Tuning the Characteristic of the Applied Potential To Improve AC-iEK Separations of Microparticles
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DOI：
10.1021/acs.analchem.3c00995
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发表时间：
2023-06-21
影响因子：
7.4
通讯作者：
Lapizco-Encinas, Blanca H.
中科院分区：
文献类型：
--
作者：
Ahamed, Nuzhet Nihaar Nasir;Mendiola-Escobedo, Carlos A.;Lapizco-Encinas, Blanca H.
目前迫切需要开发快速可靠的微粒和细胞评估方法，而电动（EK）现象可以以低成本和无标记的方式满足这一需求。本研究结合建模和experimentationto分离相同大小（5.1 μ m），形状（球形），和基板材料（聚苯乙烯）的微粒的二元混合物，但与差异的颗粒zeta电位只有14 mV，通过施加直流（DC）偏置的低频交流（AC）电压在绝缘体为基础的EK（iEK）系统。进行了四个不同的分离，以系统地研究微调所施加电压的三个主要特性：频率，幅度和直流偏置的效果。结果表明，微调每个参数改善了分离从初始分离分辨率R（s）= 0.5到最终分辨率R（s）= 3.1的完全微调分离。该分离方法在保留时间上表现出良好的重现性，实验重复性之间的变化范围为6 - 26%。目前的研究表明，有可能延长iEK系统的限制，加上仔细微调的直流偏置低频交流电压，以执行歧视性的微米级颗粒分离。
Thereis an immediate need for the development of rapid and reliablemethods for microparticle and cell assessments, and electrokinetic(EK) phenomena can be exploited to meet that need in a low cost andlabel-free fashion. The present study combines modeling and experimentationto separate a binary mixture of microparticles of the same size (5.1 & mu;m), shape (spherical), and substrate material (polystyrene),but with a difference in particle zeta potentials of only & SIM;14mV, by applying direct current (DC)-biased low-frequency alternatingcurrent (AC) voltages in an insulator-based-EK (iEK) system. Fourdistinct separations were carried out to systematically study theeffect of fine-tuning each of the three main characteristics of theapplied voltage: frequency, amplitude, and DC bias. The results indicatethat fine-tuning each parameter improved the separation from an initialseparation resolution R (s) = 0.5 to a finalresolution R (s) = 3.1 of the fully fine-tunedseparation. The separation method exhibited fair reproducibility inretention time with variations ranging from 6 to 26% between experimentalrepetitions. The present study demonstrates the potential to extendthe limits of iEK systems coupled with carefully fine-tuned DC-biasedlow-frequency AC voltages to perform discriminatory micron-sized particleseparations.