TRANSCRIPTIONAL ACTIVATION BY THE SV40 AP-1 RECOGNITION ELEMENT IN YEAST IS MEDIATED BY A FACTOR SIMILAR TO AP-1 THAT IS DISTINCT FROM GCN4
TRANSCRIPTIONAL ACTIVATION BY THE SV40 AP-1 RECOGNITION ELEMENT IN YEAST IS MEDIATED BY A FACTOR SIMILAR TO AP-1 THAT IS DISTINCT FROM GCN4
复制标题
DOI：
10.1016/0092-8674(88)90393-5
复制
发表时间：
1988-04-22
期刊：
影响因子：
64.5
通讯作者：
PARKER, CS
中科院分区：
文献类型：
--
作者：
HARSHMAN, KD;MOYEROWLEY, WS;PARKER, CS
哺乳动物转录因子AP-1的共有识别元件与转录激活因子GCN 4非常相似。在这里，我们表明，AP-1识别元件（ARE）中发现的SV 40增强子可以激活转录从异源启动子在酿酒酵母。然而，这种激活不依赖于GCN 4的存在，如gCN 4酵母菌株中ARE依赖性转录所证明的。一个以前未知的酵母转录因子，可能是负责这种激活被确定和高度纯化。命名为雅普-1的酵母因子与哺乳动物AP-1具有非常相似的生化和DNA结合特征。这些数据表明，酵母和哺乳动物AP-1是进化保守的，可能在功能上相关。同样值得注意的是，GCN 4可以以近似相等的亲和力结合GCN 4识别元件（GCRE）和ARE;然而，雅普-1对GCRE的亲和力比ARE低得多，这表明雅普-1可以在体内区分这些元件。
The consensus recognition element for the mammalian transcription factor AP-1 is very similar to that of the transcriptional activator GCN4. Here, we show that the AP-1 recognition element (ARE) found in the SV40 enhancer can activate transcription from a heterologous promoter in Saccharomyces cerevisiae. This activation, however, is not dependent on the presence of GCN4 as evidenced by ARE-dependent transcription in a gcn4 yeast strain. A previously unknown yeast transcription factor that is probably responsible for this activation was identified and highly purified. The yeast factor, designated yAP-1, shares remarkably similar biochemical and DNA-binding characteristics with mammalian AP-1. These data suggest that the yeast and mammalian AP-1 are evolutionarily conserved and perhaps functionally related. Also noteworthy is that GCN4 can bind to a GCN4 recognition element (GCRE) and to the ARE with approximately equal affinities; yAP-1, however, has a much lower affinity for the GCRE than the ARE, suggesting that yAP-1 can discriminate between these elements in vivo.